应用PCR-RFLP和芯片生物分析系统鉴别河豚鱼品种  

Identification of Puffer Species by PCR-RFLP Analysis and Chip Bio-analysis System

作　　者：陈双雅[1] 陈文炳[2] 张津[3] 陈伟玲[1] 徐敦明[1] 周昱[1] 

机构地区：[1]厦门出入境检验检疫局,福建厦门361026 [2]福建出入境检验检疫局,福建福州350001 [3]安捷伦科技有限公司,北京100102

出　　处：《食品科学》2012年第22期200-202,共3页Food Science

基　　金：国家质量监督检验检疫总局科技项目(2011IK245;2008IK175)

摘　　要：应用聚合酶链式反应-限制性片段长度多态分析和芯片生物分析系统建立5种河豚的分子生物学品种鉴定新方法。首先提取鱼的DNA进行细胞色素b基因的PCR扩增,然后用DdeⅠ、HaeⅢ和NlaⅢ三种限制性内切酶进行酶切,在Agilent DNA1000芯片上对酶切片段进行分离。该方法可成功区分5个河豚鱼品种,是一种快速、简便、有效的鱼类品种鉴定分析手段。Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） analysis and chip bio-analysis system were used to identify five puffer species.A 464-bp fragment of mitochondrial cytochrome b gene was amplified by PCR and the products were digested with the restriction endonuclease DdeⅠ,Hae Ⅲ and Nla Ⅲ,respectively.The resulting fragments were further resolved on DNA chip.Five puffer species were successfully identified by this method.In conclusion,PCR-RFLP and chip bio-analysis system could provide a fast,easy,automatic and reliable analysis approach.

关 键 词：河豚 PCR-RFLP 芯片生物分析系统 品种鉴定 

分 类 号：S917.4[农业科学—水产科学]