卧式生物反应器固定化少根根霉发酵生产脂肪酶  

Production of lipase by immobilized cells from Rhizopus arrhizus in a horizontal bioreactor

作　　者：冀颐之[1,2] 龚平[1] 赵有玺[1] 王群群[1] 谭天伟[3] 

机构地区：[1]北京联合大学生物化学工程学院,北京100023 [2]天津科技大学生物工程学院天津市工业微生物重点实验室,天津300222 [3]北京化工大学教育部生物炼制工程中心,北京100029

出　　处：《工业微生物》2012年第6期8-13,共6页Industrial Microbiology

基　　金：国家"863"项目(No.2002AA514030);国家"十五"攻关项目(No.2001BA708B02208);国家自然科学基金项目(No.2107600C);中石化项目(No.202059);北京市属高等学校人才强教计划资助项目-中青年骨干人才项目(PHR-201108426);北京市委组织部优秀人才培养资助项目(2012D005022000007)

摘　　要：以聚氨酯为固定化载体,采用6 L卧式生物反应器进行少根根霉脂肪酶的发酵研究。考察了固定化面积、通气量、转速、装液量等参数对脂肪酶生产的影响,确定了最佳的发酵条件为:聚氨酯固定床为载体,通气量5 L/min、转速40 r/min、装液量3.5 L。在此条件下,研究了分批发酵代谢规律,获得了最高脂肪酶酶活95 U/mL,单位时间产率2021 U/(h·L)。重复批次发酵,重复3批次,获得平均酶活95 U/mL,最高酶活可达110 LU/mL,单位时间产率3476 U/(h·L),是批次反应的1.72倍。试验证明以聚氨酯固定床为载体的少根根霉菌体具有良好的重复使用率。Production of lipase by immobilized cells from Rhizopus arrhizus was studied in a horizontal bioreactor with polyurethane foam as a porous biomass support. The effects of immobilized areas, aeration rate, rotating rate and volume of broth on the lipase activity were investigated. The optimum fermentation conditions obtained were as follows： aeration rate 5 L/min, rotation speed 40 r/min, and 3.5 L medium with the cylindrical bracket in 6 L horizontal bioreactor. The highest lipase activity of 95 U/mL and the lipase productivity of 2021 U/（ h ~ L） were achieved in batch fermentation under the optimized fermentation conditions. Three repeated batch fermentation were carried out in 6 L horizontal bioreactor. The highest lipase activity of 110 U/mL and the average lipase activity of 95 U/mL were achieved. The lipase productivity increased from 2021 U/（ h·L） in batch fermentation to 3476 U/（ h·L） in repeated batch fermentation, which was 1.72 times as high as that in batch fermentation. It was found that immobilized cells showed high stability for repeated use.

关 键 词：少根根霉 脂肪酶 固定化 卧式生物反应器 重复批次发酵 

分 类 号：TQ925.6[轻工技术与工程—发酵工程]