水产品中副溶血性弧菌脉冲场凝胶电泳分型  

Subspecies typing of Vibrio parahaemolyticus in aquatic products with pulsed-field gel electrophoresis

作　　者：张昕[1] 汪琦[1] 张惠媛[1] 陈广全[1] 张捷[1] 

机构地区：[1]北京出入境检验检疫局检验检疫技术中心食品实验室,100026

出　　处：《中国公共卫生》2012年第12期1666-1668,共3页Chinese Journal of Public Health

基　　金：国家质量监督检验检疫总局科技计划项目(2008IK139)

摘　　要：目的采用脉冲场凝胶电泳(PFGE)对水产品中副溶血性弧菌进行分子分型。方法采用PFGE技术,对80株分离自11个国家和中国浙江省和山东省的水产品中的副溶血性弧菌进行分子分型;基因组DNA用Not I和Sfi I 2种酶进行消化。结果 Not I和Sfi I 2种内切酶均得到68种带型,综合系统树图中分成70种带型;Not I和Sfi I的分辨率分别为78.8%和76.3%;14株来自加拿大的菌株分聚成相似度100%的4组,其中有4株菌和10株菌分别聚成相似度91.8%和93.3%的2组;挪威和新西兰分别有3株和2株菌相似度为100%;其他国家的菌株无与本国相同的菌株。结论 2种酶均适合于副溶血性弧菌的PFGE分型,Not I的效果略好于Sfi I;菌株间的亲缘关系与来源地无明显关联,但具有毒性基因的菌株更易在系统发育树上处于较近的分枝上。Objective To assess subspecies typing of Vibrio parahaemolyticus strains from aquatic products with pulsed-field gel electrophoresis（PEGE）.Methods A total of 80 selected isolates obtained from aquatic products were characterized with PFGE method.Genomic DNA was digested with restriction endonucleases NotI and SfiI.Results The isolates were grouped into 68 PFGE patterns by NotI and SfiI.Moreover,there were 70 PFGE patterns after compared with the results of the two restriction enzymes.The discrimination power of NotI was 78.8% and that of SfiI was 76.3%.Fourteen strains were from Canada four strain groups according to the Dice coefficient of 100%,thereinto,the Dice coefficient of four strains was 91.8% and the Dice coefficient of ten strains was 93.3%.Three strains form Norway and two strains from New Zealand belonged to 2 groups,with the Dice coefficient of 100%.There were no other same strains detected.Conclusion The results indicate that PFGE with both NotI and SfiI could be used in discriminant analysis for Vibrio parahaemolyticus,but NotI is more effective.The clustering analysis showes a clear disagreement among the strains and their sources.Furthermore,the strains with virulence genes are included in the same or contiguous clusters.

关 键 词：副溶血性弧菌 脉冲场凝胶电泳(PFGE) 分子分型 

分 类 号：R183.4[医药卫生—流行病学]