畜禽粪污土壤中产纤维素酶真菌的筛选及产酶条件研究  

Screening of cellulase-producting fungi from livestock feces soil and optimization of fermentation conditions

作　　者：葛永怡[1] 蒙禹势 李祝[1] 刘永霞 郭晓沙[1] 张传萍[1] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州省环境科学研究设计院,贵州贵阳550002

出　　处：《广东农业科学》2012年第21期175-177,共3页Guangdong Agricultural Sciences

基　　金：毕节市循环经济研究院专项资金(毕循专合字[2010]ZK009号);贵州省科技厅社会发展攻关(黔科合SZ字[2009]3005)

摘　　要：从畜禽养殖场土壤中分离纯化、初筛到3株产纤维素酶的真菌菌株,通过摇瓶复筛,获得1株酶活性较高的菌株HX-02,经分类鉴定确定为青霉属(Penicillium sp.),并对其发酵条件进行了研究,结果表明,以2%麸皮、3%酵母膏、0.05%MgSO4.7H2O、0.1%KH2PO4为液体发酵培养基,装液量20%、起始pH值6.0的条件下,以30℃、140 r/min发酵5 d,纤维素酶活可达到71.72(±0.53)IU/mL。Three cellulase-producing fungi strains were isolated from different livestock feces soil samples, and one fungi HX-02 which could product higher cellulase was identified as Penicillium sp.after selecting by liquid fermentation. In addition,the optimum ferment conditions of the HX-02 strain were as follows：liquid ferment medium including wheat bran 2%,yeast extract 3%,MgSO4-7H20 0.05 % ,KH2PO4 0.1% ,substrateas 20%, temperature 30℃,initial pH 6.0,140 r/m, culture time 5 days, and the cellulase activity（CMCase） reached 71.72 （±0.53）IU/mL.

关 键 词：畜禽粪污 纤维素酶 筛选 发酵 

分 类 号：S872[农业科学—畜牧兽医]