甘薯叶中SOD的分离纯化研究  

Separation and purification of SOD from sweet potato leaves

作　　者：潘明[1] 王世宽[1] 谢仁有[1] 洪玉程[1] 

机构地区：[1]四川理工学院生物工程学院,自贡643000

出　　处：《食品科技》2012年第12期239-242,共4页Food Science and Technology

基　　金：四川省科技厅自然科学基金项目(10ZA139)

摘　　要：通过比较几种提取的方法对纯化的倍数和酶活收率的影响,得到了甘薯叶上清液的较优提取纯化方法,即采用热变性,有机物丙酮沉淀及凝胶过滤纯化相结合的方法。热变性最佳时间为15min、温度为60℃,加入丙酮的量为其粗酶液的1.4倍。经凝胶过滤纯化,最终SOD酶活收率为61.5%,蛋白质去除率为97.9%,比活力达到1126.87U/mg,纯化倍数为30.32。By compared of several extraction methods＇ impact for the purify multiple and the enzyme activity yield, the optimal extraction method which combine the thermal denaturation, organic acetone precipitation and gel filtration purification was obtained. The thermal denaturation time was 15 minutes, the temperature was 60 ℃; the amount of acetone was 1.4 times to the crude enzyme solution. By gel filtration purification, the enzyme activity yield was 61.5%, the removal yield to protein was 97.9%, the specific activity was 1126.87 U/mg, the purify multiple was 30.32.

关 键 词：甘薯叶 SOD 分离纯化 比活力 

分 类 号：R284.2[医药卫生—中药学]