KLF4和SPARC在非小细胞肺癌中的表达及其相关性研究  

Correlation of KLF4 and SPARC Expression with the Clinical Characteristics of Non-small Cell Lung Cancer

作　　者：张志平[1,2] 王洲 刘相燕[1] 史墨[1] 陈钢[1] 张波[3] 李哲[3] 宋亮[1] 

机构地区：[1]山东大学附属省立医院胸外科,济南250021 [2]山东大学附属济南市中心医院胸外科,济南250013 [3]山东大学附属济南市中心医院病理科,济南250013

出　　处：《中国肺癌杂志》2012年第12期720-724,共5页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金(No.81172161);中国博士后科学基金(No.20100481272)资助~~

摘　　要：背景与目的已有研究证实KLF4基因(Krüppel-like factor4)和富含半胱氨酸的酸性分泌蛋白(se-creted protein acidic and rich in cysteine,SPARC)与肿瘤的发生发展密切相关。本研究旨在检测KLF4和SPARC蛋白在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达,并结合临床病理特征来探讨KLF4和SPARC的临床意义及相关性。方法应用免疫组织化学方法检测89例NSCLC组织及正常肺组织中KLF4和SPARC的表达。结果 KLF4在癌旁正常肺组织阳性表达率为88.8%,NSCLC组织为42.7%(P<0.05);有、无淋巴结转移者的KLF4阳性表达率分别为31.3%和56.1%(P<0.05);KLF4的表达与肿瘤临床分期有关(P<0.05),随着临床分期等级的增加,KLF4表达呈现递减趋势。SPARC在NSCLC组织的阳性表达率为70.8%,癌旁正常肺组织为7.9%(P<0.05);低、高分化癌的SPARC阳性表达率无统计学差异(P>0.05);有、无淋巴结转移者的SPARC阳性表达率分别为81.3%和58.5%(P<0.05);其表达与肿瘤的临床分期相关(P<0.05)。KLF4和SPARC的表达均与患者的性别、年龄和肿瘤大小无关(P>0.05)。SPARC和KLF4在NSCLC中的表达呈负相关(r=-0.245,P<0.05)。结论 KLF4低表达及SPARC的过表达与NSCLC的发生及其生物学行为密切相关,可能作为NSCLC诊断及分期预后的指标。Background and objective It has been proven that the development and biological behavior of lung carcinoma is affected by a number of signaling pathway elements. The expression levels of Kr/ippel-like factor 4 （KLF4） and secreted protein acidic and rich in cysteine （SPARC） were correlated with tumorigenesis and prognosis. This study aims to investigate KLF4 and SPARC expression and their correlation with the clinical characteristics of non-small cell lung cancer （NSCLC）. Methods KLF4 and SPARC expression was examined immunohistochemically in NSCLC and normal lung tissues from 89 patients. Results SPARC expression was increased in the tumor specimens compared with the control tissue （70.8% vs 7.9%, P〈0.0S）, whereas KLF4 protein was reduced compared with that in the control tissue （42.7% vs 88.8%, P〈0.05）. KLF4 expression was significantly correlated with lymph node metastasis and clinical stage （P〈0.05）. KLF4 expression in NSCLC decreased with the increasing clinical stage. The positive rate of SPARC expression in NSCLC with lymph node metastasis was significantly higher than that without lymph node metastasis （81.3% vs58.5%, P〈0.05）. Lung carcinomas in stages I and II disease had significantly lower rates of positive SPARC expression than that in stages III and IV diseases （P〈0.05）. Both were not related to age, sex, and tumor size （P〉0.05）. KLF4 expression was negatively correlated with SPARC expression in NSCLC （r=-0.245, P〈0.05）. Conclusion The SPARC overexpression may play an important role in the initiation and development of NSCLC, whereas KLF4 inhibits this process. These proteins may be used as marker for evaluating the biological characteristics and clinical stages of NSCLC.

关 键 词：肺肿瘤 KLF4 SPARC 

分 类 号：R734.2[医药卫生—肿瘤]