一个具有启动子功能的酵母DNA自主复制起始区  

A DNA Autonomous Replication Origin with Promoter Function in Yeast

作　　者：霍克克[1] 张迪[1] 唐南筠[1] 陆身枫 李育阳[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室,上海200433

出　　处：《高技术通讯》2000年第5期11-15,共5页Chinese High Technology Letters

基　　金：863计划资助项目!( 963 10 2 11 0 2 0 2 )

摘　　要：对乳酸克鲁维酵母 (Kluyveromyceslactis)天然线性双链DNA质粒 pGKL1中一个有自主复制起始功能的 6 38bp片段进行了研究。序列分析结果显示该片段中存在两个高度保守的酵母ACS (ARSconsensussequence)序列和多个ARS box序列。缺失突变分析表明该片段的自主复制功能与其ACS密切相关 ,并且证实它的两个ACS都有功能 ,可以各自独立发挥作用。据此提出 10bp序列 (5’ TCATAATATA/T 3’)是酵母K lactis的一种ACS。质粒转化试验发现该片段不仅能赋予重组质粒在酵母中自主复制的能力 ,而且能使无启动子的报告基因在酵母中表达。以上结果首次发现了一个兼有启动子功能的酵母DNA自主复制起始区 ,这对于研究真核DNA复制与转录机制及两者之间的关系有重要的意义。An autonomous replication region with promoter function was discovered from a fragment of the linear double strand DNA plasmid pGKL1 that naturally exists in the yeast Kluyveromyces lactis The result of DNA sequencing shown that two highly conserved yeast ACS(ARS consensus sequence)and some ARS boxes were located within the 638bp of region The fragment can not only enable a recombinant plasmid autonomously replicate, but also make a promoter less report gene express in yeast Deletion analysis revealed that the ARS activity was closely related to the two ACSs Both the ACSs were functional, and each can act independently Therefore, the sequence 5’ TCATAATATA/T 3’can be thought as an ACS of K lactis The results of this work demonstrated that a DNA autonomous replication region with the promoter function in yeast was firstly identified, which is significant to research on the mechanism of replication and transcription in eukaryote

关 键 词：复制 转录 启动子 酵母菌 DNA 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]