一株微囊藻毒素降解菌的筛选及鉴定  

Screening and identification of microcystins degrading bacterial strain

作　　者：谢维[1] 吴涓[1] 李玉成[1] 何席伟[1] 杜洋[1] 王光云[1] 

机构地区：[1]安徽大学资源与环境工程学院,合肥230601

出　　处：《生物学杂志》2012年第6期35-38,共4页Journal of Biology

基　　金：国家自然科学基金资助项目(40972092);安徽省自然科学基金资助项目(090415203);安徽大学大学生创新实验基金赞助项目(101035715)

摘　　要：从蓝藻爆发期间的巢湖底泥中筛选微囊藻毒素降解菌,为水体中藻毒素-LR(MC-LR)污染的生物治理提供有效的菌源。以MC-LR为唯一碳氮源,利用富集驯化培养技术分离筛选MC-LR降解菌,并对其进行形态观察、生理生化实验及16S rRNA序列分析鉴定。从巢湖底泥中分离得到一株藻毒素-LR降解菌株M6,生理生化实验及分子鉴定结果均表明,该菌株为蜡状芽孢杆菌(Bacillus cereus)。分离出的MC-LR降解菌为蜡状芽孢杆菌,该菌株对MC-LR有较高的降解能力。To provide effective microorganisms for the treatment of water polluted by microcystins（MCs）,the strains with capablity of biodegrading microcystin LR（MC-LR） were isolated from the sediment of Chaohu Lake.The enrichment culture using MC-LR as the sole carbon source was utilized to isolate the microcystin-degrading strains.The isolated strain was identified according to the observation of morphologica,the physiological and chemical tests and the analysis of 16S rRNA gene sequences.Strain M6 effectively degraded MC-LR.Phylogenetic analysis based on 16S rRNA gene showed the similarity of 99% to Bacillus cereus.So,M6 was identified as Bacillus cereus and experimental results showed the strain M6 had the higher degrading capacity for MC-LR.

关 键 词：微囊藻毒素-LR 筛选 鉴定 16S RRNA 微生物降解 

分 类 号：Q93-331[生物学—微生物学] X172[环境科学与工程—环境科学]