大豆多糖对S_(180)荷瘤小鼠免疫调节作用的研究  

Immunomodulation of soybean polysaccharide on S_(180)-bearing mice

作　　者：张秀娟[1,2,3] 孙向菊[3] 严松[4] 李其成[3] 刘思含[1,2] 张鑫[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心生物安全评价研究所,黑龙江哈尔滨150076 [2]国家教育部抗肿瘤天然药物教育部工程研究中心,黑龙江哈尔滨150076 [3]哈尔滨商业大学药学院,黑龙江哈尔滨150076 [4]黑龙江省农业科学院食品加工研究所,黑龙江哈尔滨150086

出　　处：《药物评价研究》2012年第6期420-422,共3页Drug Evaluation Research

基　　金：黑龙江省教育厅项目(12521133)

摘　　要：目的从免疫学角度探讨大豆多糖对荷瘤小鼠抗肿瘤作用的机制。方法采用分光光度计测定大豆多糖对S180荷瘤小鼠巨噬细胞一氧化氮生成量的影响,利用酶标仪测定大豆多糖对S180荷瘤小鼠腹腔巨噬细胞吞噬功能的影响,定量溶血分光光度法检测B细胞功能。结果与对照组比较,大豆多糖各组均能显著提高小鼠巨噬细胞的吞噬活性,同时能明显提高荷瘤小鼠巨噬细胞产生一氧化氮的能力,并能使体内B淋巴细胞数量增加而提高抗体生成量。结论大豆多糖通过调节荷瘤小鼠免疫功能发挥抗肿瘤作用。Objective To study the antitumor effects of soybean polysaccharide（SPS） on S180-bearing mice and the mechanism from the point of immunological view.Methods The effect of SPS on the nitric oxide（NO） generation by macrophage in S180-bearing mice was detected by spectrophotometer;ELIASA was used to determine the effect of SPS on phagocytosis of macrophage;The function of B-lymphocytes was detected by quantitative haemolysis specteophotomeric method.Results Compared with the control group,SPS could significantly enhance the phagocytosis of macrophage and increase the NO generation of macrophage as well as the content of B-lymphocytes in S180-bearing mice,so as to increase the generation of antibodies.Conclusion SPS could play its antitumor role by regulating the immune function of mice.

关 键 词：大豆多糖 抗肿瘤 免疫调节 

分 类 号：R979.1[医药卫生—药品] R979.5[医药卫生—药学]