质谱技术分析多发性骨髓瘤患者血清差异蛋白  

Detection of Serum Differential Expression Protein by MALDI-TOF-MS in Patients with Multiple Myeloma

作　　者：赵冠飞[1] 房亚哲[1] 吴琳[1] 翟玉华[1] 张洪涛[1] 陈文明[1] 王清涛[1] 

机构地区：[1]首都医科大学附属北京朝阳医院检验科,北京100020

出　　处：《标记免疫分析与临床》2012年第6期338-343,共6页Labeled Immunoassays and Clinical Medicine

基　　金：国家自然科学基金资助项目(30872391)

摘　　要：目的应用双向电泳(2-DE)和质谱技术分析多发性骨髓瘤(MM)患者血清中的差异表达蛋白,寻找MM特异性蛋白标记物。方法收集MM患者5例、其他血液系统恶性肿瘤患者及正常对照者血清各10例,三组样本等体积混合,去除高丰度白蛋白和IgG,应用2-DE分离3组血清样本,凝胶经银染显色和图像数据分析识别差异蛋白质点,以基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)对差异蛋白质点进行鉴定。结果通过凝胶软件分析MM与疾病对照组及正常对照组血清2-DE图谱,共得到51个三倍以上差异蛋白点,对上述差异蛋白进行MALDI-TOF-MS质谱分析,共鉴定出22种阳性蛋白,其中13种蛋白表达上调,包括高表达蛋白如丝氨酸/苏氨酸蛋白激酶RIO1亚型2、Rab相关蛋白Rab37亚型2、HP蛋白、锌指结构α2糖蛋白等;9种蛋白表达下调,包括α2-HS-糖蛋白、转铁蛋白、多聚素-1等。结论 MM组与疾病对照组和正常对照组间存在差异表达的蛋白质,这些差异蛋白有助于MM早期诊断和鉴别诊断,并为MM发病机制的进一步研究提供线索。Objective Using proteomics technology to analysis and identify the serum differentially expression proteins in patients with multiple myeloma and to search the possible biomarkers of multiple myeloma for clinical diagnosis. Methods The serum samples were collected from patients with multiple myeloma, other blood malig- nant tumors and healthy control group. The albumin and IgG in serum samples removed and the proteins in ser- um were separated by two-dimensional gel were electrophoresis （2DE）. After silver staining, images were cap- tured by scanner, and then edited and matched by Imagemaster 2D platinum analysis software. The differential- ly expressed proteins were identified by peptide mass tion time of flight mass spectrometry （ MALDI-TOF- fingerprint base on matrix-assisted laser desorpfion ioniza- MS）. Results Compared with controls, 51 protein spots were differently expressed in serum of patients with multiple myeloma, and 22 proteins were identified. Among them, 13 kinds of protein including serine/threonine-protein kinase RIOK1 isoform 2, ras-related protein Rab- 37 isoform 2, immunoglobulin lambda light chain VLJ region, keratin 1, actinin alpha 1 isoform 3, HP protein, Human Zinc-Alpha-2-Glycoprotein, cytokeratin 9 were up-regulated. 9 kinds of protein including Alpha-2- HS- glyeoprotein, poly （ ADP- ribose ） polymerase family member 1, indoleamine- pyrrole 2,3 dioxygenase, Phosphodiesterase 4D interacting protein, Human Apolipoprotein A-I, myosin, light polypeptide 6B, muhime- fin-1 were down-regulated. Conclusion The 2DE and MALDI-TOF-MS could be used to detect the differential expression proteins in serum of patients with multiple myeloma, and these proteins could be used as potential disease biomarkers for understand the mechanism of the development and progression of multiple myeloma.

关 键 词：多发性骨髓瘤 双向凝胶电泳 质谱鉴定 差异蛋白 

分 类 号：R733.3[医药卫生—肿瘤]