脑室注射组胺对下丘脑室旁核促皮质素释放激素神经元的作用  

Effect of intraventricular injection of histamine on corticotropin releasing hormone neurons in paraventricular nucleus of hypothalamus

作　　者：张静[1] 高国全[2] 王竹立[1] 李朝阳 卢光启[1] 

机构地区：[1]中山医科大学生理教研室,广东广州510089 [2]中山医科大学生化教研室,广东广州510089

出　　处：《中国病理生理杂志》2000年第6期512-515,共4页Chinese Journal of Pathophysiology

摘　　要：目的 :观察第三脑室注射组胺对下丘脑室旁核促皮质素释放激素 (CRH)神经元活动的影响。方法 :Fos癌蛋白免疫组化LSAB法结合双抗原标记法 ;半定量逆转录聚合酶链反应 (RT -PCR)方法。结果 :第三脑室注射组胺后 ,(1)下丘脑室旁核Fos阳性神经元数目明显增加 (P <0 .0 5 ) ;(2 )室旁核内的Fos阳性神经元中约有 31 78%同时呈CRH阳性反应 ;(3)室旁核CRHmRNA含量明显升高 ,且有量效关系。结论 :中枢组胺可以激活下丘脑室旁核的CRH神经元 ,并使CRH基因表达增加。AIM:To study the effect of intraventricular injection(icv) of histamine(HA) on corticotropin releasing hormone (CRH) neurons activity in paraventricular nucleus (PVN) of hypothalamus. METHOD: Fos ontogenic protein immunohistochemistry and double antigen immunohistochemistry method; semiquantitative reverse transcription-polymerase chain reaction(RT-PCR) method were used. RESULTS: After intraventricular injection of HA, (1) number of Fos immunnoreactive positive neurons in PVN increased significantly; (2) double labeling experiments showed that there were 31 78%±1 59% of Fos immunoreactive cells in PVN expressed CRH simultaneously; (3) the CRHmRNA level in PVN was increased in a dose-dependent manner. CONCLUSION: Central histamine activated CRH neurons in PVN of hypothalamus and increase the expression of CRH gene.

关 键 词：组胺 下丘脑室旁核 促肾上腺皮质素释放激素 

分 类 号：R338.27[医药卫生—人体生理学]