建立显微分离细胞技术并检测胃癌细胞内p53基因突变  

A microdissection technique for the molecular diagnosis of p53 gene mutations in gastric carcinoma

作　　者：邵春奎[1] 苏祖兰[1] 朱正纲 尹浩然 林言箴 王瑞年[3] 

机构地区：[1]中山医科大学附属三院病理科,广东广州510630 [2]上海市消化外科研究所,上海200025 [3]上海第二医科大学病理教研室,上海200025

出　　处：《癌症》2000年第6期534-537,共4页Chinese Journal of Cancer

基　　金：上海市医学领先学科基金!(No:94-Ⅲ005)

摘　　要：目的：获取较纯的微量胃癌细胞并检测ｐ５３基因的突变。方法：在显微镜下，直接分离出胃癌组织中微小癌灶内细胞，并应用单链构象多态（ＳＳＣＰ）技术分析该微量癌细胞中ｐ５３基因的突变。结果：在１７１例胃癌组织的石蜡标本中，有 ７０例获得可靠结果包括 ６例早期胃癌和 ６４例进展期胃癌，该方法总成功率为 ４１.０％。结论：显微分离细胞技术十分有利于观察癌前病变、原位癌及浸润癌的基因变化；该方法直接证明早期胃癌细胞中ｐ５３基因发生了突变。Objective: To obtain pure gastric carcinoma cells for the molecular diagnosis of p53 gene mutations in gastric carcinoma. Methods: A microdissection technique was developed which consisted in picking out minute cell populations from paraffinembedded tissue sections under light microscopy and analyzing p53 gene mutations in the minute carcinoma cell populations by SSCP. Results: Of 171 cases of gastric carcinoma, 70 were analyzed successfully with a successful rate of 41.0%. including 6 cases of early gastric carcinoma (EGC) and 64 cases of advanced gastric carcinoma (AGC). Conclusions: The microdissection technique proposed proved to be useful in gene studies of premalignant. in situ as well as invasive cancerous lesions and the method could directely demonstrate p53 gene mutation in EGC.

关 键 词：显微分离细胞技术 P53基因 胃肿瘤 基因突变 

分 类 号：R735.2[医药卫生—肿瘤]