人食管鳞状细胞癌细胞株hsa-miR-143/145基因簇表达生物学信息的分析  

Expression of hsa-miR-143/145 cluster in human ESCC cell line and bioinformatics analysis

作　　者：蒋森[1,2] 杨晓娣[3] 王秀芳[1,4] 鲍子超[4] 付海龙[1,4] 杜云翔[2] 赵亚萍[4] 

机构地区：[1]蚌埠医学院研究生部,安徽蚌埠233000 [2]中国人民解放军第八二医院肿瘤科,江苏淮安223001 [3]上海交通大学医学院附属瑞金医院消化科,上海200025 [4]中国人民解放军第八二医院检验科,江苏淮安223001

出　　处：《中华肿瘤防治杂志》2012年第23期1790-1794,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：江苏省淮安市产学研合作促进计划专项资金(HAC201026)

摘　　要：目的:探讨hsa-miR-143/145基因簇在人食管鳞状细胞癌(ESCC)细胞株中的表达,并对其生物学特征以及功能进行预测分析。方法:通过实时荧光定量PCR法,检测人ESCC细胞株KYSE-150和正常食管上皮细胞株Het-1A中hsa-miR-143/145基因簇的相对含量;应用miRBase获取并分析hsa-miR-143/145基因簇序列特征并比较其同源性大小;应用TargetScan 6.1、PicTar及miRanda预测hsa-miR-143/145基因簇的靶基因,取三者预测结果的交集,并结合已证实的靶基因进行功能注释(GO)和通路富集分析。结果:人ESCC细胞株KYSE-150中hsa-miR-143表达水平为0.000 2±0.000 5,显著低于正常食管上皮细胞Het-1A的0.000 6±0.000 7,t=10.301,P=0.001;hsa-miR-145的表达水平为0.000 5±0.000 1,显著低于正常食管上皮细胞Het-1A的0.001 2±0.000 2,t=6.139,P=0.004。应用3种靶标预测软件预测hsa-miR-143和hsa-miR-145的靶基因,取交集有119个基因,结合已证实的靶标基因,共224个。hsa-miR-143/145基因簇在21个物种上都显示了很好的同源性,其靶基因显著富集在癌症通路、与癌症发生相关的信号通路以及心肌通路中。结论:Hsa-miR-143/145基因簇可能参与ESCC的发病机制,其预测靶基因集合富集于多个生物学过程并且显著富集于癌症通路,为后续Hsa-miR-143/145基因簇生物学功能的研究奠定了基础。OBJECTIVE：To investigate the expression of hsa miR 143/145 cluster in human ESCC cell line,and pre dict its biological character and function. METHODS： The expression of hsa-miR-143/145 cluster was assessed by real time quantitative polymerase chain reaction （qPCR）. MiRBase database was applied to retrieval the sequence of hsa-miR-143/145 clus ter and its sequence homology. The target genes of hsa-miR-143/145 cluster were predicted using TargetScan6.1 ,PicTar and mi Randa,and the intersection of the three results as gene set was analyzed by gene ontology and pathway analysis. RESULIS： The expression levels of hsa-miR-143 and hsa-miR 145 were significantly lower in human ESCC cell line KYSE-150 than those in hu- man esophageal epithelial cell Het-lA, respectively [（0. 000 2 ± 0. 000 5） vs （0. 000 6 ± 0. 000 7）, t= 10. 301, P = 0. 001 ; （0. 000 5±0. 000 1） vs （0. 001 2±0. 000 2） ,t=6. 139,P=0. 004]. Totally 119 genes predicted to be hsa-miR-143/145 cluster targets by three target prediction programs,combined target gene which has been confirmed,totally 224. Hsa-miR-143/145 cluster was considered much homologous in 21 species. The target genes set of hsa-miR-143/145 cluster mainly existed in signaling path- ways related to tumor and myocardium. CONCLUSIONS： Hsa-miR-143/145 cluster is probably involved in ESCC pathogenesis. The target genes set of hsa-miR-143/145 cluster are significantly enriched in tumor related with signaling pathways for laying the foundation for the further study in human esophageal squamous cell carcinoma.

关 键 词：食管肿瘤 hsa—miR-143 145基因簇 细胞系 计算生物学 

分 类 号：R735.1[医药卫生—肿瘤]