顶空固相微萃取-气相色谱-质谱法分析芝麻香精的挥发性成分  

GC-MS Analysis of Volatile Components of Sesame Essence with Head-Space Solid Phase Micro-extraction

作　　者：秦早[1] 高桂园[1] 杨冉[1] 范璐[2] 李建军[1] 屈凌波[1,2] 黄纪念 张勋 

机构地区：[1]郑州大学化学系,郑州450000 [2]河南工业大学化学化工学院,郑州450000 [3]河南农业科学院,郑州450000

出　　处：《理化检验（化学分册）》2012年第12期1417-1420,1423,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：河南省重大公益项目(091100910200)

摘　　要：采用顶空固相微萃取-气相色谱-质谱法分离和鉴定芝麻香精中挥发性成分,用归一化法测定其相对含量。为使固相微萃取达到更高的效率,选用50/30μm DVB-CAR-PDMS的固相萃取头,萃取温度及时间为60℃和1h。两种芝麻香精中分别鉴定出31和28种香气成分,其中含量较高的几类物质分别为吡嗪类、呋喃类、吡啶类、酮类和酚类物质。1号香精中的主要香气化合物为:1,6-二氢-咪唑并[4,5-d]咪唑、甲基吡嗪、3,5-二甲基苯酚、糠醛、2,6-二甲基-4-氨基吡啶和5-甲基-2-呋喃甲醛。2号香精中的主要香气化合物为:2,3-二甲基吡嗪、2-糠酸糠酯、4-氨基吡啶、3,5-二甲基苯酚、乙酰基吡嗪和糠醛。Volatile components in sesame essence were separated and identified by headspace solid phase micro-extraction （HS-SPME） and GC-MS, and their relative contents （in %） were found by the method of normalization. To attain a higher efficiency in HS-SPME, 50/30 μm DVB-CAR-PDMS fiber as coating was selected for micro-extractor and the extraction was carried out at 60℃ for 1 h. Thirty-one compounds in sesame essence 1 ~ and 28 compounds in sesame essence 2# were identified among which main compounds were pyrazine, furan, pyridine, ketones and phenols. The major compounds found in sesame essence 1# were as follows： 1, ＆dihydro- imidazo[4, 5-d] imidazole, methyl-pyrazine, 3, 5-dimethyl-phenol, furfural, 2, 6-dimethyl-4-pyridinamine and 5- methyl-2-furancarboxaldehyde; the major compounds found in sesame essence 2~ were as follows： 2, 3-dimethyl- pyrazine, 2-furfurylthiol, 4 pyridinamine, 3,5-dimethyl-phenol, acetylpyrazine and furfural, respectively.

关 键 词：顶空固相微萃取 气相色谱-质谱法 芝麻香精 挥发性成分 

分 类 号：O657.63[理学—分析化学]