高效液相色谱-荧光法测定肉类塑料包装物中7种环境雌激素  

HPLC-FLD Determination of Seven Environmental Estrogens in Meat Packaging Materials

作　　者：武中平[1] 卢剑[1] 高巍[1] 张晓强[1] 王莉[1] 车文军[1] 

机构地区：[1]江苏省产品质量监督检验研究院,江苏南京210007

出　　处：《肉类研究》2012年第11期23-26,共4页Meat Research

基　　金：国家质量监督检验检疫总局科技计划资助项目(2008QK114)

摘　　要：采用高效液相色谱-荧光法对肉类塑料包装物中7种环境雌激素(雌三醇、双酚A、对壬基酚、对特辛基苯酚、特丁基对苯二酚、苯酚、17-β-雌二醇)含量进行测定。样品用二氯甲烷溶解、甲醇沉淀后,经C18固相萃取小柱净化,用高效液相色谱、荧光检测器对肉类塑料包装物中7种环境雌激素的测定。结果表明:该方法样品平均加标回收率为90.4%～105.7%,相对标准偏差为1.63%～8.15%;塑料食品包装物中11种环境雌激素的检出限为0.071～0.220mg/kg。An HPLC-FLD method was developed to determine 7 environmental estrogens in met packaging materials. Samples were dissolved in dichloromethane and precipitated by adding methanol. The filtrate was cleaned up on C18 SPE column and separated by high performance liquid chromatography, and detected with a fluorescence detector （FLD）. Average spike recoveries between 90,4% and 105.7% and relative standard deviations （RSD） between 1.63% and 8.15% were obtained for spiked samples. The limits of detection （LOD） of the HPLC method were between 0.071 mg/kg and 0.22 mg/kg.

关 键 词：高效液相色谱-荧光 肉类塑料包装物 环境雌激素 酚类化合物 固相萃取 

分 类 号：TS206.4[轻工技术与工程—食品科学] O657.3[轻工技术与工程—食品科学与工程]