应用单核苷酸多态性技术对食品中大肠埃希氏菌的地理溯源  

SINGLE-NUCLEOTIDE POLYMORPHISM INTERROGATION OF GEOGRAPHICAL SOURCE OF ESCHERICHIA COLI IN FOOD

作　　者：赵宏[1] 尹静[1] 曲鹏[2] 李君文[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050 [2]天津出入境检验检疫局动植物与食品检测中心

出　　处：《解放军预防医学杂志》2012年第6期394-397,共4页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：国家自然科学基金青年基金项目(No.3090119);国家"863"项目(No.2009AA03Z332);天津市科技计划项目(No.11ZCKFSF01100)

摘　　要：目的利用大肠埃希氏菌持家基因中高分辨力单核苷酸多态性(SNP)位点对不同地理来源食品中的大肠埃希氏菌进行地理溯源,为追溯、控制食源性感染提供技术保障。方法根据大肠埃希氏菌多位点基因序列分型(MLST)数据库中序列信息,利用SNP分析软件Minimum SNPs测算得到大肠埃希氏菌4个持家基因中的12个高分辨力SNP位点。利用这些位点组合对来源于12个国家或地区的食品中分离出的253株大肠埃希氏菌进行分析。结果得到了大肠埃希氏菌特异性的地理标记;盲样分析证明洲际溯源准确率为80%,国家或地区溯源准确率为60%。结论持家基因中的高分辨力SNP位点组合具有对不同地理来源大肠埃希氏菌进行追溯的能力。Objective To study the geographical source of the E.coli in foods by highly discriminary single-nucleotide polymorphism（SNP） technology,so as to provide the technical basis for control of E coli infection from food.Methods A set of 12 SNPs,with a discrimination value（Simpson＇s index of diversity（D）） of 0.86,was determined using the Minimum SNPs software,based on sequences of housekeeping genes from the E.coli multilocus sequence typing（MLST） database.Two hundred and fifty-three E.coli isolates from various geographical sources in food were sereened and analysed.Results Some Geography-specific SNP profiles were found and verified by blind test.The accuracy of source tracing of E.coli isolates from different continents was up to 80%;and the accuracy of source tracing from differcnt countries was up to 60%.Conclusion SNP sets in conservative housekeeping genes of E.coli can identify a specific geographical origin of E.coli.

关 键 词：大肠埃希氏菌 持家基因 单核苷酸多态性 溯源 食品 分子分型 

分 类 号：Q524[生物学—生物化学]