产油脂海洋微藻的筛选、鉴定及Fe^(3+)对其生长和油脂积累的影响  

Screening and identification of oil-rich marine microalgae and effects of Fe^(3+) on its growth and oil accumulation

作　　者：孙漫[1,2] 聂娟[1,2] 袁维道[1,2] 张福特[1,2] 方哲[2] 黄慧琴[2] 鲍时翔[2] 

机构地区：[1]海南大学农学院,海口570228 [2]中国热带农业科学院热带生物技术研究所,农业部热带作物生物学与遗传资源利用重点实验室,海口571101

出　　处：《中国油脂》2012年第12期70-73,共4页China Oils and Fats

基　　金："973"计划项目(2011CB200900);中央级公益性科研院所基金项目(1630012011004);中央级公益性科研院所基本科研业务费专项资金(1630052012002)

摘　　要：采用尼罗红染色和油脂提取相结合的方法,从105株海洋微藻中,筛选并鉴定了1株油脂产量较高的微藻琴式菱形藻(Psammodictyon panduriforme),研究了Fe3+对其生长和油脂积累的影响。结果表明,添加Fe3+有利于Psammodictyon panduriforme的生长和油脂积累,在Fe3+浓度为4×10-4mol/L时,其生物量最大(0.65 g/L),油脂含量最高(49.07%),此时的油脂产量也最高,达到了318.96 mg/L,是不加Fe3+培养的6.15倍。Psammodictyon panduriforme was screened and identified from 105 strains of marine microalgae for its higher oil yield by nile red dyeing combining with oil extraction. Effects of different concentrations of Fe3＋ on cell growth and oil accumulation of Psammodictyon panduriforme were investigated. The results showed that the biomass and oil content of Psammodictyon panduriforme were improved by adding Fe3＋ to culture medium. When the concentration of Fe3＋ was 4 ×10-4 tool/L, it obtained highest biomass of 0.65 g/L and highest oil content of 49.07%. Besides, it had highest oil yield of 318.96 mg/L which was 6.15 times as high as that withoutF3＋.

关 键 词：琴式菱形藻 筛选 鉴定 FE3+ 尼罗红染色 油脂提取 产油脂海洋微藻 

分 类 号：TE667[石油与天然气工程—油气加工工程]