CAPN1基因4685位点与肉质嫩度相关性研究  

Close Association between 4685 Loci on CAPN1 Gene and Meat Tenderness

作　　者：姜成国 全世元 曹阳[3] 张国梁[3] 董宝池[3] 金海国[3] 张立春[3] 

机构地区：[1]延边州畜牧总站,吉林延吉133000 [2]敦化市畜牧站,吉林敦化133700 [3]吉林省农业科学院,吉林长春130030

出　　处：《安徽农业科学》2013年第1期161-163,共3页Journal of Anhui Agricultural Sciences

基　　金：国家高技术研究发展计划(863)项目(2008AA101010);国家"十一五"科技支撑项目(2008BADB2B01);国家科技基础条件平台家养动物种质资源平台(2012)项目

摘　　要：[目的]探索延边黄牛与草原红牛钙蛋白酶I(CAPN1)基因4685位点对肉质嫩度的影响。[方法]采用PCR-RFLP技术对草原红牛、延边黄牛CAPN1基因第14内含子区4685位点进行基因多态性与肉质嫩度进行分析,验证该位点在吉林省地方品种牛中的作用。[结果]该位点与肉质嫩度相关检测指标(蒸煮损失、肌纤维直径、剪切力、滴水损失等)密切相关,均表现为T等位基因的存在能够显著提高个体的嫩度水平,但该位点与pH无关。4685位点与屠宰性状(净肉重、胴体重、净肉率等)无关,仅与眼肌面积存在一定的相关性。[结论]延边黄牛与草原红牛CAPN1基因4685位点与肉质嫩度存在密切相关。[ Objective] The paper was to explore the impact of 4685 loci on CAPN1 gene of Yanbian yellow cattle and red steppe cattle on meat tenderness. [ Method] 4685 loci in 14th intron region of CAPN1 gene of Yanbian yellow cattle and red steppe cattle was conducted analysis of gene polymorphism and meat tenderness by PCR-RFLP technology, and the effect of the loci on Jilin local cattle breeds was verified. [ Result] The loci had close relationship with related detection indexes of meat tenderness （cooking loss, muscle fibre diameter, shear force and drip loss）, and the existence of T allele could significantly increase the tenderness level of individuals, but the loci was not associated to pH value. 4685 locl was not associated to carcass traits （carcass weight, net meat weight and carcass yield ratio）, which only had certain correlation with eye muscle area. [ Conclusion] 4685 loci on CAPN1 gene of Yanbian yellow cattle and red steppe cattle had close association with meat ten- derness.

关 键 词：延边黄牛 草原红牛 CAPN1基因 嫩度 

分 类 号：S823.81[农业科学—畜牧学]