梅花鹿胎盘免疫调节因子制备工艺  

Preparation of Immunoregulatory Factors from Sika Deer Placenta

作　　者：孙辞[1] 边媛媛[1,2] 吕永通[1] 胡风庆[1] 

机构地区：[1]辽宁大学生命科学院生物材料与生物制药实验室,辽宁沈阳110036 [2]江南大学食品学院,江苏无锡214000

出　　处：《食品科学》2012年第24期107-110,共4页Food Science

基　　金：沈阳市发展与改革委员会高技术研发基金项目(2010-16);辽宁省教育厅基金项目(L2010150)

摘　　要：以梅花鹿鹿胎盘作为原料,制备具有生物活性的小分子活性物质——鹿胎免疫调节因子。通过正交试验优化超声功率、固液比、超声时间和丙酮量等因素,获得最优化鹿胎提取液制备工艺为超声功率400W、固液比1:2、超声时间120s、(提取液:丙酮比1:3),此条件下鹿胎提取液多肽质量浓度可达30.35mg/mL。之后,利用超滤法处理鹿胎提取液,可得到微黄色透明液体即为鹿胎免疫调节因子,主要含多肽(3.7±0.04)mg/mL、核酸(3.3±0.07)μg/mL、多糖(0.54±0.002)mg/mL,且超滤法显著优于透析法。Immunoregulatory factors as bioactive small molecules were prepared from sika deer placenta homogenate using ultrasonic-assisted acetone extraction. Process conditions such as ultrasonic power, homogenate-to-normal saline ratio, utrasonic treatment time and homogenate-to-acetone ratio were optimized using an orthogonal array design to be 400 W, 1：1 （g/mL）, 120 min and 1：3 （V/V）, respectively. The polypeptide concentration of the extract obtained under these conditions was 30.35 mg/mL. The extract was further ultrafiltrated and a clear yellowish liquid containing immunoregulatory factors was obtained. The liquid contained mainly （3.7±0.04） mg/mL peptides, （3.3 ± 0.07） μg/mL nucleic acid and （0.54 ±0.002） mg/mL polysaccharides, whereas smaller amounts of polypeptides, nucleic acid and polysaccharides were found in the retentate of the extract after dialysis.

关 键 词：梅花鹿胎盘 免疫调节因子 正交试验 制备工艺 

分 类 号：TS201.1[轻工技术与工程—食品科学]