前列腺癌p53基因的点突变  

Point Mutation of p53 Gene in Prostate Carcinomas

作　　者：黄纲雄[1] 陈碧芬[1] 晋雯[1] 高美钦[1] 

机构地区：[1]福建医科大学病理解剖学教研室,福州350004

出　　处：《福建医科大学学报》2000年第2期135-137,共3页Journal of Fujian Medical University

摘　　要：目的　检测前列腺癌组织中 p5 3基因突变的发生率 ,探讨其与前列腺癌发生的关系。　方法　应用多聚酶链反应 -限制性片段长度多态性方法 ,检测前列腺癌组织中 p5 3基因第 4号外显子 72位密码子的点突变。　结果　前列腺癌 DNA样本中检出 4号外显子 p5 3基因点突变 44 % ,其中杂合性突变 36 % ,纯合性突变 8%。对照组前列腺良性增生未见 p5 3基因变化。　结论　前列腺癌发生与 p5 3基因突变有一定关系 ,应用 PCR和 Bstu I酶切多态性分析 ,对检测前列腺癌 p5 3基因第 4号外显子 72位编码的点突变是一较特异的方法。Objective\ To determine how the point mutation of p53 was correlated with the prostate cancer.\ Methods\ Polymerization chain reaction(PCR)\|restriction fragment length polymorphisms(RFLP) technique was used to detect p53 gene and analyze the mutation of p53 gene exon 4 condon 72 in 25 prostate carcinomas, DUl45 cell line and benign prostatic hypertrophy(BPH) as well as normal prostate tissues.\ Results\ P53 gene exon 4 was present in the chromosome DNA of all samples.\ The mutation p53 gene exon 4 condon 72 was present in 11 out of 25 carcinoma cases(44%), among them the mutation of homologosity in 2(8%), heterozygosity in 9(36%) and for the wild type of p53 with no mutation in 14(56%).\ No mutations were detected in those obtained from DU145 cell and 8 samples with non\|tumors.\ Conclusion\ These findings suggest that p53 may be a target of chromosome 17 deletions and that this gene may play a role in the pathogenesis of prostate cancer.\ RFLP with Bstu I analysis of the p53 gene codon 72 may be a useful and direct technique to detect the point mutation of p53 gene in prostate cancer.\;

关 键 词：前列腺癌 P53基因 基因突变 聚合酶链反应 

分 类 号：R737.25[医药卫生—肿瘤]