Semaphorin 3A及其受体Neuropilin-1在人舌鳞状细胞癌/大鼠背根神经节共培养中对轴突芽生的影响  

The role Semaphorin 3A and its receptor Neuropilin-1 play in mediating the sprout of axons in co-culture of SCC25 and rats' dorsal root ganglion

作　　者：唐子春[1] 王子露[2] 宋晓萌[2] 朱江[2] 吴煜农[2] 

机构地区：[1]铜陵市人民医院口腔科,安徽铜陵244000 [2]南京医科大学口腔医学研究所.附属口腔医院口腔颌面外科,江苏南京210029

出　　处：《口腔生物医学》2012年第4期189-192,200,共5页Oral Biomedicine

基　　金：江苏高校优势学科建设工程资助项目;江苏省自然科学基金(BK2010531)

摘　　要：目的:构建人舌鳞状细胞癌/大鼠背根神经节(dorsal root ganglion,DRG)体外共培养的实验模型,研究神经轴突导向因子Semaphorin 3A(Sema3A)及其受体Neuropilin-1(NRP-1)在肿瘤排斥神经的现象中可能发挥的作用。方法:鉴定Sema3A在人舌鳞状细胞癌细胞系SCC25中的表达以及NRP-1在大鼠DRG神经元中的表达,建立人舌鳞状细胞癌/大鼠DRG体外共培养的实验模型,针对NRP-1靶点进行特异性拮抗,观察SCC25排斥DRG轴突生长的程度变化。结果:免疫荧光结果显示,Sema3A在SCC25中表达,而NRP-1在大鼠DRG神经元中也呈阳性表达;共培养实验结果表明,DRG组织块中NRP-1被特异性拮抗后,SCC25对DRG轴突的排斥程度较对照组明显减弱。结论:Sema3A及其受体NRP-1在SCC25排斥大鼠DRG轴突生长过程中起着重要作用。Objective：To investigate the role of the neural axon targeting factor, Semaphorin 3A （Sema3A） and its receptor Neuropilin-1 （NRP-1） in the process of tumors rejecting nerve tissues, we established the model of co-culturing the human squamous carcinoma cell 25 （SCC25） with rat dorsal root ganglions （DRG） in vitro. Methods：We determined the expression of Sema3A and NRP-1 respectively in SCC25 and rat DRG, then established the in vitro model of co-culturing the human squamous carcinoma cell with rat DRG, followed by observing the change of SCC25 rejecting the sprouting of DRG axons after specifically antagonising NRP-1. Results：The resuits of immunofluence confirmed the expression of Sema3 A in SCC25 and the positive expression of NRP-1 in rat DRG, and the co-culture model showed that, after specifically antagonising NRP-1, the rejection of SCC25 to DRG axons diminished signicantly compared to the eontrol group. Conclusions：Sema3A with its reeeptor NRP-1 may play an important role in SCC25 rejeeting the sprouting of DRG

关 键 词：舌鳞状细胞癌 Semaphorin-3A 背根神经节 NEUROPILIN-1 共培养 

分 类 号：R739.86[医药卫生—肿瘤]