RP-HPLC法同时测定石榴皮中4种多酚类成分的含量  

Content Determination of 4 Polyphenols in Punica granatum by RP-HPLC

作　　者：刘振平[1,2] 陈祥贵[1] 彭海燕[1] 杨文宇[1] 杨潇[1] 何宇新 李玉锋[1] 

机构地区：[1]西华大学生物工程学院食品生物技术四川省高校重点实验室,成都610039 [2]重庆安全技术职业学院,重庆404000

出　　处：《中国药房》2013年第3期238-240,共3页China Pharmacy

基　　金：国家科技部星火计划重点项目资助(No.2010GA810002);四川省科技攻关项目资助(No.05SG011-021)

摘　　要：目的:建立同时检测石榴皮中没食子酸、石榴皮鞣素、安石榴苷和鞣花酸含量的方法。方法:采用反相高效液相色谱法。色谱柱为ArcusEP-C18(250mm×4.6mm,5μm),流动相为甲醇-0.1%三氟乙酸(梯度洗脱),流速为1.0ml/min,检测波长为254nm(没食子酸)、377nm(石榴皮鞣素、安石榴苷和鞣花酸)。结果:没食子酸、石榴皮鞣素、安石榴苷和鞣花酸的进样量分别在0.020～0.320、0.038～0.608、0.074～1.184、0.039～0.624μg范围内与各自峰面积积分值呈良好线性关系(r分别为0.9997、0.9971、0.9978、0.9994);平均加样回收率分别为93.22%、95.35%、98.00%、99.84%,RSD分别为2.75%、2.28%、2.11%、1.82%(n均为6)。结论:本方法精密、可靠,可作为检测石榴皮中没食子酸、石榴皮鞣素、安石榴苷和鞣花酸4种有效成分的方法。OBJECTIVE：To develop a method for the content determination of gallic acid,punicalin,punicalagin and ellagic acid in Punica granatum.METHODS：RP-HPLC method was used.The determination was carried out on Arcus EP-C 18（250 mm× 4.6 mm,5 μm）with mobile phase consisted of methanol-0.1%TFA（gradient elution）at a flow rate of 1.0 ml/min.The detection wavelength of gallic acid was at 254 nm,and that of punicalin,punicalagin and ellagic acid were at 377 nm.RESULTS：The linear range was 0.020-0.320 μg for gallic acid（r=0.999 7）,0.038-0.608 μg for punicalin（r=0.997 1）,0.074-1.184 μg for punicalagin（r=0.997 8）,0.039-0.624 μg for ellagic acid（r=0.999 4）.The average recoveries were 93.22%（RSD=2.75%,n=6）,95.35%（RSD=2.28%,n=6）,98.00%（RSD=2.11%,n=6）and 99.84%（RSD=1.82%,n=6）,respectively.CONCLUSION：The method is accurate,reliable and suitable for the content determination of gallic acid,punicalin,punicalagin and ellagic acid in P.granatum.

关 键 词：石榴皮 没食子酸 石榴皮鞣素 安石榴苷 鞣花酸 反相高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]