水稻矮秆突变体Zj88d的鉴定与基因定位  

Characterization and Gene Mapping of a Dwarf Mutant Zj88d in Rice

作　　者：纪现军[1,2] 叶胜海[2] 周涯[2,3] 修芬连[2,3] 邓晓梅[1,2] 尚海漩[2,3] 刘继云[2,3] 陈萍萍[2,3] 李小华[1,2] 金庆生[2] 张小明[2] 

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310036 [2]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021 [3]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《中国水稻科学》2013年第1期35-40,共6页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(31071207;31100142);浙江省科技项目(2007C12902;2010R50033);公益性行业(农业)科研专项(201103007)

摘　　要：用甲基磺酸乙酯诱变处理常规粳稻浙粳88,获得了矮秆水稻突变体Zj88d,多代自交稳定遗传。遗传分析表明突变体Zj88d的矮秆性状受一对隐性核基因控制。采用图位克隆方法,将目的基因定位在水稻第7染色体短臂末端7-44w和RM5344间的603kb区间。排查该区间包含的100个候选基因,发现已克隆的矮秆基因OSH15也位于其中。测序结果显示,突变体Zj88d中的OSH15基因在第2内含子最末位发生"g→a"单碱基突变。The stably inherited dwarf mutant Zj88d was produced by treating the dry seeds of japonica rice variety Zhejing 88 with ethyl methylsulfonate（EMS）.Genetic analysis of the dwarf mutant Zj88d revealed that this trait is controlled by one pair of recessive nuclear gene.The gene was located in the interval of 603 kb between 7-44w and RM5344 markers on the short arm of chromosome 7 by map-based cloning.The dwarf gene ＂OSH15＂ was identified by screening one hundred candidate genes on the target chromosomal segment.Sequencing results further revealed that Zj88d gene has a single-base substitution（g to a） in the last nucleotide of intron 2 of OSH15 allele.

关 键 词：水稻 矮秆 等位基因 单碱基突变 选择性剪切 

分 类 号：S511[农业科学—作物学]