浒苔多糖功能饮料对2型糖尿病大鼠糖脂代谢的影响及其机制  

Effect of polysaccharides in enteromorpha functional beverage on glycolipid metabolism of type 2 diabetes and its mechanism

作　　者：林文庭[1] 廖冬冬[1] 陈达妙[1] 

机构地区：[1]福建医科大学公共卫生学院营养与保健医学系,福建福州350108

出　　处：《中国老年学杂志》2013年第1期93-96,共4页Chinese Journal of Gerontology

基　　金：国家级星火计划项目(2011GA720014);福建省自然科学基金项目(2011J0118)

摘　　要：目的观察浒苔多糖功能饮料(EB)对2型糖尿病大鼠脂代谢影响及其作用机制。方法高糖高脂饲料喂养,加小剂量注射STZ(30 mg/kg)造模,EB灌胃干预28 d。测定大鼠周空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和胰岛素(INS)水平并计算胰岛素敏感指数(ISI),检测大鼠肝脏组织中葡萄糖激酶(GCK)、胰岛素受体(InsR)和脂肪组织中葡萄糖转运体(GLUT)-4、脂联素(APN)mRNA的表达水平。结果与模型组比较,EB各剂量组FBG和INS较低,ISI较高,EB高、中剂量组TC、TG、LDL-C均明显较低,EB高、低剂量组MDA较低、SOD和GSH-Px均较高,EB各剂量组肝脏InsR mRNA的表达较低,脂肪GLUT-4 mRNA表达较高,EB高、中剂量组肝脏GCK mRNA和脂肪APN mRNA表达量均较高(P<0.05或P<0.01)。结论 EB可通过上调脂肪组织中APN的表达,从而增强胰岛素敏感性,改善IR;也可通过上调脂肪组织GLUT-4和肝脏组织葡萄糖代谢关键酶GCK基因的表达,促进葡萄糖的转运和代谢,改善糖脂代谢异常。Objective To observe the effects of polysaccharides in enteromorpha functional beverage （EB） on adjusting the glyco- lipid metabolism of type 2 diabetic （T2DM） rats and discuss its possible mechanisms. Methods Rats model of T2DM were established by a high sugar and fat diet with intraperitoneal injection of STZ （30 mg/kg）, and then given various doses of EB for 28 days. Fasting blood glucose （FBG）, total cholesterol （TC）, triglyceride （TG）, high density lipoprotein cholesterol （HDL-C）, low density lipoprotein choles-terol （ LDL-C）, malondialdehyde （ MDA）, superoxide dismutase （ SOD）, glutathione peroxidase （ GSH-Px）, insulin （INS） were measured weekly and insulin sensitive index （ISI） was calculated. Expressions of glucokinase （GCK） mRNA, insulin receptor （InsR） mRNA in liver tissue and GLUT-4 mRNA, adiponectin （APN） mRNA in fat tissue were detected by RT-PCR. Results Compared with model group, FBG and INS in all EB intervention groups were lower, but ISI was higher. TC, TG and LDL-C in high and medium dose EB groups were reduced significantly. MDA was lower, SOD and GSI-I-Px were higher in high and low dose groups. Expression of InsR mRNA in liver was lower and GLUT-4 mRNA in adipose was higher in all EB intervention groups. Expressions of APN mRNA in adipose and GCK mRNA in liver were higher in high and medium dose groups （P 〈 0. 05 or P 〈 0. 01 ）. Conclusions EB can up-regulate APN expression in adipose to increase insulin sensitivity, improve the IR, also can up-regulate GLUT-4 and GCK expressions in adipose and liver tissue to promote the transport and metabolism of glucose.

关 键 词：浒苔多糖功能饮料 血糖 血脂 APN GCK GLUT-4 

分 类 号：R151.3[医药卫生—营养与食品卫生学]