灰树花子实体与发酵菌丝体挥发性化合物分析  

Analysis of volatile compounds in fruiting bodies and submergedly cultured mycelia of Grifola frondosa

作　　者：杨生兵[1] 陆震鸣[1] 耿燕[1] 许泓瑜[1] 徐国华 史劲松[1] 许正宏[1] 

机构地区：[1]江南大学药学院,江苏无锡214122 [2]江苏神华药业有限公司,江苏淮安211600

出　　处：《菌物学报》2013年第1期103-113,共11页Mycosystema

基　　金：江苏省自然科学基金(No.BK2010142);国家"863"重点项目子课题(No.2007AA021506);教育部新世纪人才支持计划(No.NCET-07-0380)

摘　　要：分别采用固体栽培法和液体发酵法对同一灰树花菌株进行培养,获得灰树花子实体和发酵菌丝体,并采用顶空固相微萃取(HS-SPME)和气相色谱-质谱(GC-MS)分析技术对子实体和菌丝体的挥发性化合物进行了分析比较。结果表明,灰树花子实体和发酵菌丝体分别含有62种和94种挥发性物质,其中37种为相同物质,分别占总挥发性物质的86.81%和84.28%。灰树花子实体中含有醇(14种)、酮(13种)、醛(12种)、酸(7种)、酯(5种)等物质,主要以酯类(42.80%)、醛类(35.14%)物质为主,其中乙酸乙酯的相对含量达到了42.57%。发酵菌丝体中主要含有醛(22种)、酮(17种)、醇(16种)、酯(13种)、酸(12种)等挥发性物质,其中醛类、酯类、酸类、醇类分别占47.0%、10.90%、7.48%和5.94%。异戊醛和地衣酚的含量分别达到了23.31%、15.41%。Fruiting bodies and mycelia of Grifola frondosa were produced by solid-state and submerged cultures, respectively. The volatile compounds of fruiting bodies and mycelia were analyzed by headspace solid-phase microextraction （HS-SPME） combined with gas chromatography-mass spectrometry （GC-MS）. The results showed that fruiting bodies and mycelia of Grifolafrondosa, respectively, contained 62 and 94 volatile compounds. Hereinto, 37 compounds in both fruiting bodies and mycelia accounted for 86.81% and 84.28% of total volatile substances, respectively. The fruiting bodies mainlycontained alcohol （14）, ketones （13）, formaldehyde （12）, acid （7）, esters （5）, and so on. Esters （42.80%） and aldehydes （35.14%） were the main volatile compounds of fruiting bodies, and the relative content of ethyl acetate was 42.57%. The mycelia mainly contained formaldehyde （22）, ketones （17）, alcohol （16）, esters （13）, acid （12） and so on, of which aldehydes, esters, acids and alcohols, respectively, accounted for 47.0%, 10.90%, 7.48% and 5.94%. The content of isovaleraldehyde and lichen phenol reached 23.31% and 15.41%, respectively.

关 键 词：灰树花 挥发性化合物 顶空固相微萃取 气相色谱-质谱分析 

分 类 号：S646[农业科学—蔬菜学]