壳聚糖微球的制备及其固定化木瓜蛋白酶的研究  

Studies on Preparation of Chitosan Microspheres and Immobilization of Papain on Them

作　　者：李红[1] 王炜军[1] 徐凤彩[1] 

机构地区：[1]华南农业大学生物技术学院,广东广州510642

出　　处：《华南农业大学学报》2000年第2期49-53,共5页Journal of South China Agricultural University

摘　　要：用来源丰富且廉价 ,并具有许多优良生物特性的壳聚糖为原料 ,以简单易行的悬浮交联法制备壳聚糖微球 ,并对其性状进行测定和分析 .结果表明 :它复合了壳聚糖与高分子微球的功能 .然后 ,以壳聚糖微球为载体 ,用吸附-交联的联合固定化方法制备固定化木瓜蛋白酶 ,并研究了木瓜蛋白酶的最佳固定化条件 .结果显示 :木瓜蛋白酶的最佳固定化条件是给酶量为 1 .92mg/g、4～ 8℃吸附 1 2h、再用体积分数为 0 .5 %的戊二醛溶液于 4～ 8℃交联 3h ,所制得的固定化木瓜蛋白酶活力达 38.49U/g ,酶活力回收率平均达 6 6 .6 0 % 。Using chitosan as a source, the chitosan microspheres were prepared by suspension crosslinking technique. Their properties were determined and analysed. The results indicated that they complexed functions of chitosan with those of macromolecule microspheres. Then as the carrier, the chitosan microspheres were used to immobilize papain by absorption crosslinking method. The optimum conditions for immobilization were studied. The results were as follows: the optimum load of enzyme was 1.92 mg enzyme/g carrier, the absorbing time was 12 h and the temperature 4～8 ℃; then interacted for 3 h at 4～8 ℃ with 0.5% glutaraldehyde. The activity and the activity recovery of the immobilized papain were 38.49 U/g carrier and 66.60% respectively.

关 键 词：壳聚糖微球 木瓜蛋白酶 固定化酶 固定化载体 

分 类 号：Q556.903[生物学—生物化学] Q814.2