国家油菜区试品系的主要营养品质及评价  

Nutritional quality and evaluation of candidate cultivars in regional trials of oilseed rape in China

作　　者：杨湄[1] 郑畅[1] 黄凤洪[1] 周琦[1] 刘昌盛[1] 王会[1] 

机构地区：[1]中国农业科学院油料作物研究所,油料脂质营养与功能湖北省重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2012年第6期604-612,共9页Chinese Journal of Oil Crop Sciences

基　　金：国家公益性行业(农业)科研专项(200903043);国家现代农业油菜产业技术体系(cars-13)

摘　　要：以50个2009/2010年度参加全国区试的油菜品系为试材,压榨取油后分别采用福林酚法测定菜籽多酚总量,高效液相色谱法测定维生素E、叶黄素、贝塔胡萝卜素含量,气相色谱法测定植物甾醇含量。结果表明,50个品系的菜籽多酚、维生素E、植物甾醇、叶黄素、贝塔胡萝卜素含量范围分别为8.02～61.90mg/100g、260.86～685.21mg/kg、608.23～1 218.51mg/100g、36.09～170.07mg/kg、2.06～4.19mg/kg,平均值分别为40.17mg/100g、476.96mg/kg、879.82mg/100g、85.40mg/kg、2.54mg/kg。品系间主要微量营养成分含量存在显著差异(p<0.01)。通过因子分析建立了油菜品系的营养品质评价模型,利用该模型可将50个品系的营养品质分为2类。通过聚类分析和随机选取的另外8个品种样品的实测结果与评分结果,验证该模型具有可行性,可用于油菜品种营养品质评价。Content of minor nutritional compositions（total phenolics,phytosterols,tocopherols,beta-carotene and lutein） of rapeseed oils from 50 Brassica napus L.lines in regional trials were determined to construct an identification model for rapeseed oil quality.Among them,total phenolics were tested by Folin-Ciocalteau colourimetric method,phytosterols contents were determined by GC,and others including tocopherols,beta-carotene and lutein were determined by RP-UPLC.Results showed that the content of oil qualities ranged from 8.02 to 61.90mg tanin acid per 100g oil for total phenolics,260.86 to 685.21mg per kilogram for tocopherols,608.23 to 1 218.51mg per 100g for phytosterols,36.09 to 170.07mg per kilogram for lutein,and 2.06 to 4.19mg per kilogram beta-carotene.Their average contents were 35.68mg per 100g,826.16mg per 100g,378.52mg per kilogram,2.39mg per kilogram,104.06mg per kilogram,and 8.21mg per kilogram respectively.There were significant differences（p 0.01） among the lines in the content of these minor components.Rapeseed nutritional quality could be evaluated by factor analysis and modeling,and be divided into two classes by cluster analysis.Cluster analysis and the grade of 8 other samples showed that the model could be used to evaluate nutritional quality of rapeseed variety.

关 键 词：油菜 菜籽多酚 维生素E 植物甾醇 叶黄素 贝塔胡萝卜素 因子分析 评价模型 

分 类 号：S565.403[农业科学—作物学]