培养基对节杆菌产β─呋喃果糖苷酶活力的影响  

The effect of culture medium on activity of β-fructofuranosidase from arthrobacter sp

作　　者：于国平[1] 迟玉杰[1] 陶菲[1] 于金魁 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《东北农业大学学报》2000年第2期184-190,共7页Journal of Northeast Agricultural University

基　　金：黑龙江省自然科学基金资助项目(1997)

摘　　要：节杆菌（ＡＳ）能够产生一种β－呋喃果糖苷酶，该酶能以蔗糖和乳糖为底物，合成低聚乳果糖（ＬＳ）。这是一种功能性低聚糖，对人体健康有益，有很好的发展前景。实验研究了培养基组成对节杆菌产β－呋喃果糖苷酶活力的影响，确定最佳培养基的配方为蔗糖４％、酵母膏１．６０％、牛肉膏０．５０％、ＫＨ_２ＰＯ_４０．２０％、（ＮＨ_４）_２ＨＰＯ_４０．４０％。Arthrobactor sp. can produce β-fructorfuranosidase. This enzyme can use sucrose and Lactose as raw materials and then sgnthesize lactosucrose. Lactosucrose is a functional oligosaccharide and is health for human body. In this paper, the effect of medium component on β-fructofuranosidase activity from AS was studied. The best medium was selected: 4%su- crose, 1.60%yerst extract, 0.50%beef extract, 0.20%KH_2PO_4, 0.40% (NH_4)_2HPO_4.

关 键 词：节杆菌 Β-呋喃果糖苷酶 低聚乳果糖 培养基 

分 类 号：TS201.3[轻工技术与工程—食品科学] TQ925[轻工技术与工程—食品科学与工程]