Varied response of the pulmonary arterial endothelium in a novel rat model of venous thromboembolism  

作　　者：JI Ying-qun FENG Min ZHANG Zhong-he LU Wei-xuan WANG Chen 

机构地区：[1]Department of Respiratory Diseases, First Affiliated Hospital ofDalian Medical University, Dalian, Liaoning 116011, China [2]Department of Respiratory Diseases, Peking Union MedicalCollege Hospital, Chinese Academy of Medical Sciences & PekingUnion Medical College Hospital, Beijing 100730, China [3]Beijing Hospital, Ministry of Health, Beijing 100730, China

出　　处：《Chinese Medical Journal》2013年第1期114-117,共4页中华医学杂志（英文版）

摘　　要：Background The experimental studies of venous thromboembolism （V-rE） as an entity and the response of the pulmonary arterial endothelium after VTE are still rare. The objective of this study was to observe changes in the pulmonary arterial endothelium using a novel rat model of VTE. Methods Rats were allocated to the VTE （n=54） or control groups （n=9）. The left femoral vein was blocked using a microvessel clip to form deep vein thrombosis （DVT）. One, four or seven-day-old thrombi were injected into the right femoral vein to induce DV-r-pulmonary thromboembolism （DVT-PTE）. The rats were sacrificed 1, 4 or 7 days later （Dn （1,4,7） Pn（1,4,7） subgroups （n=6））, and the lungs were examined using light and electron microscopy. Results On gross dissection, the rate of DVT formation was higher on day 1 （D1Pn： 100%, 18/18） than day 4 （D4Pn： 83%, 15/18; X^2=5.900, P=0.015） or day 7 （DFPn： 44%, 8/18; X2=13.846, P=0.000）. On gross dissection, the positive emboli residue rate in the pulmonary arteries was lower in the D1Pn subgroup （39%, 7/18） than the D4Pn （73%, 11/15; X2=3.915, P=0.048） and DFPn subgroups （100%, 8/8; X2=8.474, P=0.004）; however, light microscopy indicated the residual emboli rate was similar in all subgroups. Hyperplasia of the pulmonary arterial endothelium was observed 4 and 7 days after the injection of one-day-old or four-day-old thrombi. However, regions without pulmonary arterial endothelial cells and intra-elastic layers were observed one day after injection of seven-day-old thrombi. Conclusions This novel model closely simulates the clinical situations of thrombus formation and is ideal to study pulmonary endothelial cell activation. The outcome of emboli and pulmonary arterial endothelial alterations are related to the age and nature of the thrombi.Background The experimental studies of venous thromboembolism （V-rE） as an entity and the response of the pulmonary arterial endothelium after VTE are still rare. The objective of this study was to observe changes in the pulmonary arterial endothelium using a novel rat model of VTE. Methods Rats were allocated to the VTE （n=54） or control groups （n=9）. The left femoral vein was blocked using a microvessel clip to form deep vein thrombosis （DVT）. One, four or seven-day-old thrombi were injected into the right femoral vein to induce DV-r-pulmonary thromboembolism （DVT-PTE）. The rats were sacrificed 1, 4 or 7 days later （Dn （1,4,7） Pn（1,4,7） subgroups （n=6））, and the lungs were examined using light and electron microscopy. Results On gross dissection, the rate of DVT formation was higher on day 1 （D1Pn： 100%, 18/18） than day 4 （D4Pn： 83%, 15/18; X^2=5.900, P=0.015） or day 7 （DFPn： 44%, 8/18; X2=13.846, P=0.000）. On gross dissection, the positive emboli residue rate in the pulmonary arteries was lower in the D1Pn subgroup （39%, 7/18） than the D4Pn （73%, 11/15; X2=3.915, P=0.048） and DFPn subgroups （100%, 8/8; X2=8.474, P=0.004）; however, light microscopy indicated the residual emboli rate was similar in all subgroups. Hyperplasia of the pulmonary arterial endothelium was observed 4 and 7 days after the injection of one-day-old or four-day-old thrombi. However, regions without pulmonary arterial endothelial cells and intra-elastic layers were observed one day after injection of seven-day-old thrombi. Conclusions This novel model closely simulates the clinical situations of thrombus formation and is ideal to study pulmonary endothelial cell activation. The outcome of emboli and pulmonary arterial endothelial alterations are related to the age and nature of the thrombi.

关 键 词：venous thrombosis pulmonary embolism tunica endothelia PATHOLOGY disease models  animal 

分 类 号：R-332[医药卫生]