PTEN基因转染对人舌鳞癌细胞系SCC-4凋亡的影响及其作用机制  

Effect of PTEN transfection on human tongue squamous carcinoma cell line SCC-4 and its mechanism

作　　者：刘硕硕[1] 张斌[1] 郭婷婷[1] 董肖婷[1] 

机构地区：[1]辽宁医学院附属第一医院口腔科,辽宁锦州121001

出　　处：《山东大学学报（医学版）》2013年第1期42-45,共4页Journal of Shandong University:Health Sciences

基　　金：辽宁省教育厅高等学校科学研究项目(L2010315)

摘　　要：目的探讨PTEN基因转染对人舌癌细胞系SCC-4凋亡的影响及其作用机制。方法将转染PTEN的SCC-4(pEGFP-PTEN-SCC-4)细胞作为实验组,以SCC-4和pEGFP-SCC-4作为对照,流式细胞仪检测3组细胞凋亡情况;应用Western blotting法检测3组中Akt、p-Akt、Bim的表达情况。结果流式细胞仪检测结果显示,实验组细胞凋亡率比对照组明显增高(P<0.01);Western blotting结果显示,各组细胞间总Akt水平差异不明显;p-Akt在实验组中表达明显低于对照组;Bim在实验组中表达明显高于对照组。结论 PTEN转染能够诱导SCC-4的凋亡,可能机制是负调控PI3K/Akt信号通路并上调Bim的表达。Objective To explore the impact of PTEN transfection on the apoptosis of human tongue squamous carcino- ma cell line SCC-4 and its mechanism. Methods Cells transfected with PTEN （pEGFP-PTEN-SCC-4） were set as the experimental group. Cells transfected with pEGFP-SCC-4 and normal SCC-4 were the control groups. The flow cytom- etry was used to detect the apoptosis of three groups. The expressions of Akt, p-Akt and Bim proteins were detected by Western blotting. Results The apoptosis rate of the experimental group was significantly higher compared with the control groups（P 〈 0.01 ）. Western blotting showed there was no significant difference of the total Akt level between three groups. The expression of p-Akt was significantly decreased in the experimental group compared with the control groups, and the level of Bim was higher in the experimental group compared with the control groups. Conclusion PTEN transfection may induce the apoptosis of human tongue squamous cell SCC-4 by down regulating PI3K/Akt sig- naling pathway and increasing Bim expression.

关 键 词：PTEN基因 转染 舌癌细胞 PI3K/AKT Bim 凋亡 

分 类 号：R739.86[医药卫生—肿瘤]