顶空固相微萃取—气相色谱质谱联用法测定三七挥发性成分  

Analysis of Volatile Constituents from Radix Notoginseng by Headspace SolidPhase Micro-extraction Coupled with Gas Chromatography-Mass Spectrography

作　　者：李丽明[1] 任斌[2] 郭洁文[1] 陈剑梅[1] 刘若轩[1] 

机构地区：[1]广州市中医医院,广东广州510130 [2]中山大学附属第一医院,广东广州510080

出　　处：《广州中医药大学学报》2013年第1期63-67,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省社会发展领域科技计划项目(编号:83100);广东省中医药管理局基金资助项目(编号:2010274);广州市中医药中西医结合科研项目(编号:20112A011011)

摘　　要：【目的】探讨顶空固相微萃取—气相色谱质谱联用技术分析测定三七挥发性成分的可行性。【方法】利用顶空固相微萃取—气相色谱质谱联用法研究三七挥发性成分的化学成分组成。【结果】三七挥发性成分经气相色谱分离得到离子峰93个,鉴定得到83个化合物,占挥发性物质总量的95.83%。【结论】顶空固相微萃取和气相色谱质谱联用法具有简便、环保、快速、灵敏的优点,可应用于三七挥发性成分的研究。Objective To explore the feasibility of applying headspace-solid phase micro-extraction（HS-SPME） coupled with gas chromatography-mass spectrometry（GC-MS） to analyze the volatile constituents from Radix Notoginseng.Methods Volatile constituents from Radix Notoginseng was detected by HS-SPME coupled with GC-MS.Results Ninety-three peaks were obtained after the separation by the GC-MS,and among which 83 compounds were identified,accounting for 95.83% of the total volatile constituents.Conclusion HS-SPME coupled with GC-MS has the advantages of convenience,environmental protection,rapidity,and sensitivity,and is suitable for the analysis of volatile constituents from Radix Notoginseng.

关 键 词：三七 化学 顶空固相微萃取 气相色谱—质谱 挥发性成分 

分 类 号：R284.1[医药卫生—中药学]