灵芝蛋白酶解产物分析及其抗氧化活性的研究  

Analysis of Enzymetic Hydrolysis of Ganoderma lucidum Protein and Antioxidation of its Hydrolysates

作　　者：黄生权[1] 敖宏[1] 曾凡逵[2,3] 杨博[4] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640 [2]中国热带农业科学院农产品加工研究所,广东湛江524001 [3]农业部热带作物产品加工重点开放实验室,广东湛江524001 [4]华南理工大学生物科学与工程学院,广东广州510006

出　　处：《现代食品科技》2013年第1期24-28,共5页Modern Food Science and Technology

基　　金：国家自然科学基金项目(20436020);广东省科技计划项目(2009A030700007)

摘　　要：采用商品蛋白酶Alcalase和Protamex对灵芝蛋白进行水解,并对灵芝蛋白水解前后抗氧化活性的变化进行了比较。结果表明:灵芝蛋白经过蛋白酶Alcalase和Protamex水解10 h后,氨基酸态氮含量从酶解前的0.15 mg/mL分别增长到0.61 mg/mL和0.54 mg/mL;Protamex酶解得到的游离氨基酸Lys、Val、Leu、Thr、Phe、Ala、Arg、Ile、Cys、His和Try含量较高,Alcalase酶解得到的游离氨基酸Ala、Val、Glu、Leu、Arg、Try和Thr含量较高;灵芝蛋白经过蛋白酶Alcalase和Protamex水解后,清除羟自由基的能力显著增强,水解前.OH自由基清除率为28.70%,水解后分别为39.10%和33.30%;还原力亦显著增强,水解前OD700值为0.21,水解后分别为0.35和0.34。Commercial proteases Alcalase and Protamex were used to hydrolysis Ganoderma lucidum protein, the antioxidant activity of Ganoderma lucidum protein before and after hydrolysis were evaluated. The results showed that the free amino acid content increased from 0.15 mg/mL to 0.61 and 0.54 mg/mL when use Alcalase and Protamex as catalyzer. The main free amino acid were Lys, Val, Leu, Thr, Phe, Ala Arg, lie, Cys, His and Try when treated with Protamex, while the main free amino acid were Ala, Val, Glu, Leu, Arg, Try and Thr when treated with Alcalase. Before hydrolysis, the hydroxyl radical scavenging rate of Ganoderma lucidum protein was 28.70%, increased to 39.10% and 33.30% when treated with Alcalase and Protamex respectively. The reducing power also increased significantly and the OD 700 was 0.21 before hydrolysis, being increased to 0.35 and 0.34 after hydrolysis.

关 键 词：灵芝蛋白 蛋白酶 抗氧化 水解 

分 类 号：TS201.2[轻工技术与工程—食品科学]