1株毛栓菌产纤维素酶酶学性质研究  

Study on enzymological properties of cellulase produced by a Trametes Trogii strain

作　　者：贾翠英[1] 张玉辉[2] 柴高波[1] 

机构地区：[1]河南科技学院生命科技学院,河南新乡453003 [2]沈阳药科大学生命科学与生物制药学院,辽宁沈阳410016

出　　处：《河南农业大学学报》2012年第6期678-681,共4页Journal of Henan Agricultural University

基　　金：河南省科技攻关项目(112102310443)

摘　　要：对1株毛栓菌所产纤维素酶的酶学性质进行研究,主要考察了酶的最适温度、最适pH、热稳定性、pH稳定性、激活剂及抑制剂的类型.结果表明:该毛栓菌所产CMC-Na酶的最适温度为50℃,最适pH值为4.8,酶的热稳定性范围为30～50℃,60℃之后酶的活性急剧下降;pH值稳定性范围为5.0～7.0,pH值8以后酶活性急剧下降.金属离子Mn2+对CMC-Na酶有较大的激活作用,Cu2+和Mg2+对酶起抑制作用,Ca2+和Fe3+对该酶的活力几乎没有影响.Enzymological properties of cellulase produced by a Trametes trogii strain were investigated by analyzing the optimal reaction temperature, optimal pH value, thermo-stability, pH stability, the type of activator and inhibitor of enzyme. The results showed that the optimal temperature of the en- zyme was 50 ℃,the optimal pH value was 4.8. The thermal stability of the enzyme was in the range of 30 - 50 ℃,and when the temperature was over 60 ℃ , the enzyme activity would have a sharp decline. The pH stability was in the range of pH 5.0 - 7.0, and when pH value was over 8 the enzyme activity would have a sharp decline. Mn2＋ played an important role in improving the enzyme activity while Cu2＋ and Mg2＋ had inhibitory effect,and Ca2＋ and Fe3＋ had hardly any effect on the enzyme activity.

关 键 词：毛栓菌 纤维素酶 酶学性质 

分 类 号：Q556.2[生物学—生物化学]