硫磺熏蒸前后白芍HPLC-UV特征图谱的比较研究  

Comparative study on HPLC-UV specific chromatograms of Paeoniae Radix Alba before and after sulfur-fumigation

作　　者：蔡皓[1,2,3] 张科卫[1] 刘晓[1,2,3] 刘静静[1,2,3] 马晓青[1,2,3] 蔡宝昌[1,2,3,4] 李松林[5] 

机构地区：[1]南京中医药大学药学院,南京210046 [2]南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,南京210029 [3]南京中医药大学国家中医药管理局中药炮制标准重点研究室,南京210029 [4]南京海昌中药集团有限公司,南京210061 [5]江苏省中医药研究院中药分析和代谢组研究室,南京210028

出　　处：《药物分析杂志》2013年第1期128-132,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金资助项目(81173546;30940093);江苏省自然科学基金资助项目(BK2009495);江苏省国际科技合作计划资助项目(BZ2011053)

摘　　要：目的:对白芍硫磺熏蒸前后的HPLC-UV特征图谱进行比较研究,为科学评价及有效控制其质量提供可靠方法。方法:采用RP-HPLC方法,使用Hypersil ODS C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-0.5%醋酸水溶液,流速为1.0 mL·min-1,二元梯度洗脱,二极管阵列检测器,测定未经硫磺熏蒸和经硫磺熏蒸的白芍各10批,以芍药苷为参照物,检测波长为270 nm。结果:未经硫磺熏蒸的白芍中共有23个共有峰,经硫磺熏蒸的白芍中共有18个共有峰,通过与对照品的保留时间及紫外光谱比较,5、13、16、17号峰的归属分别为没食子酸、儿茶素、白芍苷、芍药苷。结论:白芍经硫磺熏蒸后,其成分发生了比较明显的变化,同时白芍在硫磺熏蒸前后,某些共有成分的含量也有不同程度的改变,硫磺熏蒸确实对白芍的质量有非常大的影响。Objective ： To comparatively analyze HPLC - UV specific chromatograms of Paeoniae Radix Alba before and after sulfur -fumigation, so as to provide reliable methods for scientifically evaluating and effectively controlling the quality of Paeoniae Radix Alba. Methods： RP - HPLC method was employed, and the chromatographic condi- tions were as follows ： Hypersil ODS C18 column （250 mm × 4.6 mm,5μm） , binary solvent delivery gradient elution of acetonitrile -water（ containing 0.5% acetic acid） as mobile phase with a flow rate of 1.0 mL . min-1, photodi- ode array detector. The detective wavelength was set at 270 nm, and ten batches of sun - dried and sulfur - fumiga- ted Paeoniae Radix Alba were respectively analyzed using paeoniflorin as the reference compound. Results：A satis- factory method for HPLC - UV specific chromatograms of Paeouiae Radix Alba before and after sulfur - fumigation was established. Totally 23 common peaks were observed in the specific ehromatogram of sun - dried Paeoniae Radix Alba ,while only 18 common peaks were found in the specific chromatogram of sulfur- fumigated Paeoniae Radix

关 键 词：白芍 硫磺熏蒸 高效液相色谱-紫外特征图谱 聚类分析 没食子酸 儿茶素 白芍苷 芍药苷 质量控制 安全监测 

分 类 号：R917[医药卫生—药物分析学]