SIRT1基因多态性与广西永福地区人群长寿的关联性  

Association between SIRT1 gene polymorphisms and longevity of populations from Yongfu region of Guangxi

作　　者：黄金[1] 孙亮[1] 刘铭[1] 周林[1] 吕泽平[2] 胡才友[2] 黄泽治 郑晨光[2] 周琳 杨泽[1] 

机构地区：[1]卫生部北京医院卫生部老年医学研究所,北京100730 [2]广西壮族自治区江滨医院 [3]广西永福县疾病控制中心 [4]北京青少年科技俱乐部

出　　处：《中华医学遗传学杂志》2013年第1期55-59,共5页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金项目（30972709,81061120527）

摘　　要：目的探讨SIRTl基因多态性与广西永福地区人群长寿的关联性。方法采用长寿一对照设计，选取广西壮族自治区永福县共500人为研究对象，包括长寿组223人、平均年龄（93．17±3．08）岁，对照组277人、平均年龄（46．92±17．12）岁。应用聚合酶链反应一高分辨熔解曲线技术结合测序验证法检测SIRTl基因上的rs3758391，rs3740051，rs2273773，rs4746720和rsl0997870位点的单核苷酸多态性（singlenucleotidepolymorphisms，SNP）的分布情况，比较长寿组与对照组人群的等位基因、基因型频率的分布特征，分析SIRT1基因多态性与长寿的关联性。结果长寿组rs4746720位点CT基因型频率高于CC和TT基因型频率，差异具有统计学意义（P=0．000，0R=2．098，95％CI：1．412-．117），其等位基因频率在两组间分布的差异无统计学意义。rs3758391、rs3740051、rs2273773位点的基因型频率和等位基因频率在长寿组和对照组间分布的差异均无统计学意义。结论S球．rI基因rs4746720单核苷酸多态性与广西永福地区长寿人群相关联。Objective To assess the association betweenSIRT1 gene polymorphisms and the longevity phenomena in Yongfu region of Guangxi. Methods In this case-control study, 500 individuals from Yongfu region of Guangxi were recruited. The subjects were divided into a longevity group （n^223, average age= 93.17±3.08 yr） and a healthy control group （n=277, average age=46.92±17.12 yr）. Polymerase chain reaction-high resolution melting curve （PCR-HRM） and DNA sequencing were used to determine the allelic and genotypic frequencies of rs3758391, rs3740051, rs2273773, rs4746720 and rs10997870 polymorphisms of SIRT1 gene in the two groups. The association between above polymorphisms and longevity was assessed. Results In the longevity group, CT genotype of the rs4746720 locus was significantly more common than CC and TT genotypes （P= 0. 000, OR= 2. 098, 95% CI： 1. 412-4. 117）. However, no significant difference was found in the allelic and genotypic frequencies of rs3758391, rs3740051 and rs2273773 between the two groups. Conclusion There is an association between rs4746720 of SIRT1 gene and longevity in Yongfu region of Guangxi.

关 键 词：SIRT1基因 单核苷酸多态性 长寿 关联分析 

分 类 号：R394[医药卫生—医学遗传学]