蝴蝶石斛兰花色表型及类黄酮成分分析  

Floral Colors of Phalaenopsis Type Dendrobium and Their Flavonoid Composition

作　　者：李崇晖[1] 任羽[1] 黄素荣[1] 黄少华[1] 杨光穗[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,农业部华南作物基因资源与种质创制重点实验室,海南儋州571737

出　　处：《园艺学报》2013年第1期107-116,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31101578);科技部科技成果推广专项(2011GB2E200006);中国热带农业科学院博士启动基金项目(PZSB1003);中央级公益性科研院所基本科研业务费专项(PZS067)

摘　　要：利用分光色差计测定了不同颜色蝴蝶石斛兰品种的花色表型,采用高效液相色谱—质谱联用技术检测花朵中类黄酮色素的组成,探讨花色表型与所含类黄酮含量之间的关系。结果表明,蝴蝶石斛兰品种花色主要分布在紫红色和粉红色区域,且唇瓣颜色比花瓣和萼片暗。花色与类黄酮含量之间存在着线性关系,而花青素苷是影响蝴蝶石斛兰花色的主要因素:总花青素苷含量与花色的明度 L*值和色相 b*值以及色相角 h 值负相关,与色相 a*值正相关。从蝴蝶石斛兰花朵中共检测出 28 种花青素苷(均为酰基化的矢车菊素–3,7,3’–三葡萄糖苷)和 19 种黄酮醇苷(苷元有槲皮素、山奈酚和异鼠李素 3 种),由此推测了蝴蝶石斛兰品种花中的类黄酮代谢途径。The floral colors of commercial Phalaenopsis type dendrobium cultivars with different colors were measured by a spectrophotometer,the flavonoid composition in the flowers were analyzed using a high performance liquid chromatography combined with mass spectrometry,then,the relationship between the floral color parameters and the flavonoid contents were discussed. The results showed that the floral colors of commercial Phalaenopsis type dendrobium cultivars mainly distributed in purple and pink region,and the color of labellum was darker than that of petal and sepal. Linear relationships between floral color parameters and flavonoid contents were discovered. The anthocyanins were the major factors affected the floral colors of Phalaenopsis type dendrobium cultivars：The total anthocyanin contents negatively correlated with lightness L＊,chromatic component b＊ and hue angle h,however,positively correlated with chromatic component a＊. Furthermore,there were 28 anthocyanins（all were acylated cyanidin-3,7,3′-triglucoside）and 19 flavonol glycosides（the aglycones were quercetin,kaempferol,and isorhamnetin）were tentatively identified,based on that,the metabolic pathway of flavonoids in Phalaenopsis type dendrobium flowers were speculated. The study also provided suggestion for the floral color breeding of Phalaenopsis type dendrobium cultivars.

关 键 词：石斛兰 蝴蝶石斛兰 花色 类黄酮 

分 类 号：S685[农业科学—观赏园艺]