利用温度调节实现新型重组菌高效转化甘油为D-乳酸  

Temperature-switched high-efficiency D-lactate production from glycerol

作　　者：田康明[1] 周丽[1] 陈献忠[1] 沈微[1] 石贵阳[2] Suren Singh 路福平[4] 王正祥[4] 

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]发酵粮食国家工程实验室,江南大学,江苏无锡214122 [3]德班理工大学生物工程与食品技术学院应用科学系 [4]天津科技大学生物工程学院,天津300457

出　　处：《生物工程学报》2013年第1期111-114,共4页Chinese Journal of Biotechnology

基　　金：国际科技合作项目(No.CS06-L11)资助~~

摘　　要：油脂水解来源的甘油将是未来发酵工业主要原料之一。文中探索D-乳酸高产大肠杆菌Escherichia coli CICIM B0013-070菌株不同培养温度下好氧与厌氧代谢甘油的特征后,建立并优化了一种新型D-乳酸变温发酵工艺,甘油到乳酸的得率由64%提高到82.6%。另外,在B0013-070中引入了温度诱导型乳酸脱氢酶的转录系统,甘油到乳酸的得率进一步提高到88.9%。Glycerol from oil hydrolysis industry is being considered as one of the abundent raw materials for fermentation industry. In present study, the aerobic and anaerobic metabolism and growth properties on glycerol by Esherichia coli C1CIM B0013-070, a D-lactate over-producing strain constructed previously, at different temperatures were investigated, followed by a novel fermentation process, named temperature-switched process, was established for D-lactate production from glycerol. Under the optimal condition, lactate yield was increased from 64.0% to 82.6%. Subsequently, the yield of D-lactate from glycerol was reached up to 88.9% while a thermo-inducible promoter was used to regulate D-lactate dehydrogenase transcription.

关 键 词：D-乳酸 甘油 变温发酵 

分 类 号：TQ921.3[轻工技术与工程—发酵工程]