阻断Ras在肝癌靶向治疗中的意义  

Significance of blocking Ras in targeted therapy of hepatocellular carcinoma

作　　者：杨件新[1] 施超[1] 

机构地区：[1]启东市人民医院普外科,江苏启东226200

出　　处：《胃肠病学和肝病学杂志》2013年第1期25-27,共3页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的探讨阻断Ras在肝癌靶向治疗中的价值。方法 MTT检测不同浓度Ras抑制剂farnesylthiosalicylic acid(FTS)对肝癌细胞生长的影响,显微镜观察细胞形态变化,流式细胞仪检测细胞凋亡,Western blot检测Erk磷酸化水平。结果 FTS可以抑制HepG2和Huh-7细胞生长,诱导HCC细胞凋亡,降低Erk磷酸化水平。结论 Ras可作为HCC治疗的潜在靶点。To investigate the role of Ras in targeted therapy of hepatocellular carcinoma （HCC）. Methods The MTT assay was used to investigate cell growth. The change of cell morphology was observed under the microscopy. Early apoptosis was detected by Annexin-V-FITC/propidium iodide double staining assay. Western blot assay was used to investigate the level of phospho-ERK. Results FTS inhibited HepG2 and Huh-7 cell growth, induced cell apoptosis, and decreased the level of phospho-ERK. Conclusion Ras may be a potential molecular target for the treatment of HCC.

关 键 词：人肝癌细胞 RAS 靶向治疗 HEPG2 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]