携带Wx-mq基因的不同类型水稻新品种(系)直链淀粉含量分析  

Analyses of amylase contents in different types of new rice varieties(lines) carrying Wx-mq gene

作　　者：于新[1] 赵庆勇[1] 赵春芳[1] 张亚东[1] 朱镇[1] 赵凌[1] 陈涛[1] 周丽慧[1] 姚姝[1] 王才林[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所,江苏省优质水稻工程技术研究中心,国家水稻改良中心南京分中心,江苏南京210014

出　　处：《江苏农业学报》2012年第6期1218-1222,共5页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省农业科技自主创新基金项目[CX(10)306];江苏省产学研前瞻性联合研究项目(BY2010134);江苏省重大科技支撑计划项目(BE2011302)

摘　　要：采用分子标记、化学方法、酶标仪、统计学等方法,对99份粳稻新品种(系)进行Wx-mq基因(低直链淀粉含量的暗胚乳突变基因)检测,提取、测定各品种(系)直链淀粉含量,并分析不同遗传背景及不同生态类型材料间直链淀粉含量的差异,旨在为低直链淀粉含量的优良食味粳稻新品种选育提供参考依据。试验结果表明:99份材料均含有Wx-mq基因;供试材料的直链淀粉含量主要分布在8%与11%之间;武香粳14与关东194杂交育成的品种(系)直链淀粉含量的变幅相对较小;不同熟期品种(系)的直链淀粉含量的变幅为中粳较晚粳大。The aim was to offer some recommendations for breeding new japonica rice varieties with good eating quality carrying low amylose content.Marker-assisted selection,chemical extraction,microplate reader and statistical methods were used to detect Wx-mq gene in 99 new rice varieties（lines） and analyze the amylose contents of materials with different genetic backgrounds and ecological types.Wx-mq gene was carried in 99 new rice varieties（lines）.The amylose contents were between 8% and 11%.The amylose contents of rice varieties（lines） derived from the combination of Wuxiangjing 14/Kanto 194 were relatively high,and the amplitude was relatively small.The amylose content differed among tested varieties（lines） with the order of medium-maturing medium japonica rice greater than medium-maturing late japonica rice,late japonica rice taking the least.The amplitude of variation of amylose content in medium japonica rice was bigger than that in late japonica rice.

关 键 词：水稻 暗胚乳突变基因 直链淀粉含量 食味品质 

分 类 号：S511.03[农业科学—作物学]