茶树品种“小雪芽”冷诱导锌指蛋白基因cDNA研究  

Study on a cDNA sequence of cold inducible zinc finger proteinin albinism tea cultivar "Xiaoxueya"

作　　者：王开荣 李娜娜[2] 陆建良[2] 郑新强[2] 梁月荣[2] 吴颖 李明 

机构地区：[1]宁波市白化茶叶专业合作社,宁波315012 [2]浙江大学茶叶研究所,杭州310058

出　　处：《茶叶》2012年第4期210-214,共5页Journal of Tea

基　　金：宁波市科技局"宁波白茶产业关键技术提升与标准化应用"项目资助

摘　　要：分析了低温诱导型新梢白化茶树品种"小雪芽"叶片低温诱导锌指蛋白基因cDNA序列。该序列长度为698bp,与大豆锌指蛋白mRNA同源性为83%,与蓖麻锌指蛋白mRNA同源性为82%;具有可编码230个氨基酸的开放阅读框。与"福鼎大白茶"冷诱导锌指蛋白cDNA序列相比,该cDNA序列在50-51位上核苷酸AT缺失,第143位的A被置换为G,第654位T缺失。其翻译的蛋白质氨基酸序列与"福鼎大白茶"同源性达到99%,但有3个位点的氨基酸变异。该基因表达丰度明显低于"福鼎大白茶"。研究认为,基因结构差异,引起表达强度和蛋白质氨基酸序列的差异,可能是引起"小雪芽"品种对低温敏感的重要因素。A cDNA sequence of cold inducible zinc finger protein in leaf of albinism tea cultivar ＂ Xiaoxueya＂ was investigated. The results shows that the cDNA sequence had 698 bp in length, with 83% and 82% identity to zinc finger protein mRNA of Glycine max and Ricinus communis respectively. It had an opening reading frame encoding 230 amino acids. Compared to that of common tea cuhivar ＂ Fudingdabai＂ , there were three loci of nucleotide deletion and one locus of nucleotide substitution. Its deduced amino acid sequence had 99% identity to that of cuhivar ＂ Fudingdabai＂ , among which there were 3 loci of amino acid substitution. The expression of cold inducible zinc finger protein in ＂ Xiaoxueya＂ ,sas significantly lower than in ＂ Fudingdabai＂. It is considered that the mutation in gene sequence resulted in the sensitivity of cultivar ＂Xiaoxueya＂ to low temperature through low expression and mutation of the cold inducible zinc finger protein.

关 键 词：茶树品种 新梢白化 基因结构 基因表达 

分 类 号：S571.1[农业科学—茶叶生产加工]