大豆异黄酮和塞来昔布预防大鼠乳腺癌的发生  

The Chemopreventive Effect of Soybean Isoflavones and Celecoxib on DMBA-induced Breast Cancer in Rats

作　　者：杨定勇[1] 赵之婧[1] 覃春阳[1] 杨立民[1] 

机构地区：[1]昆明医科大学第二附属医院胸心外科,云南昆明650101

出　　处：《昆明医科大学学报》2012年第12期22-25,共4页Journal of Kunming Medical University

基　　金：云南省应用基础研究基金资助项目(2009ZC112M)

摘　　要：目的观察大豆异黄酮和塞来昔布对7,12-二甲基苯蒽(7,12-dimethylbenz anthrancene,DMBA)诱发的大鼠乳腺癌形成的影响,并探讨其抗肿瘤机制.方法 90只SD雌大鼠随机分成DMBA组(A组)、DMBA+SOY(B组)组和DMBA+塞来昔布(C组)3组,每组30只.分别给予DMBA油剂灌胃复制大鼠乳腺癌模型,同时B组和C组分别给予含SOY及塞来昔布的饲料喂养至实验结束,观察各组大鼠乳腺癌的发生率、潜伏期及肿瘤数目.结果 B组(48.28%)和C组(48.15%)乳腺癌发生率低于A组(79.31%),差异有统计学意义(P<0.05);B组(14.71±1.90)周和C组(14.46±1.85)周乳腺癌发生的潜伏期长于A组(12.96±2.12)周,差异有统计学意义(P<0.05);B组(1.79±0.80)个和C组(1.62±0.77)个乳腺癌发生数目少于A组(2.43±0.99)个,差异有统计学意义(P<0.05).结论大豆异黄酮和塞来昔布对SD大鼠乳腺癌的发生、发展有一定的抑制作用.Objective To observe the chemopreventive effect of soybean isoflavones and celecoxib on DMBA-chemically induced breast cancer in rats, and explore the mechanisms. Methods A total of 90 rats were randomly divided into 3 groups： DMBA group （control group） , soybean isoflavones group and celecoxib group, each group contained 30 rats. DMBA wgs intragastrically given to SD female rats to build breast cancer model. Meanwhile, rats in soybean isoflavones group and celecoxib group received soybean isoflavones and celecoxib treatments, respectively. The occurrence rate, latency period, number of breast cancer were observed and analysed. Results The incidence of mammary tumors in soybean isoflavones group （48.28%） and celecoxib group （48.15%） were both lower than that in the DMBA group （79.31%）, with statistical difference （P〈 0.05 ）. The latency periods of mammary tumors in soybean isoflavones group （ 14.71 ± 1.90 weeks） and celecoxib group （14.46 ± 1.85 weeks） were longer than that in DMBA group （12.96 ± 2.12 weeks）, with statistical difference （P〈 0.05）. The tumor numbers of soybean isoflavones group （1.79 ± 0.80） and celecoxib group （1.62 ± 0.77） were less than that of DMBA group （2.43 ± 0.99） , with statistical difference （P 〈 0.05）. Conclusion Soybean isoflavones and celecoxib could effectively prevent the occurrence of DMBA-induced breast cancer in female rats.

关 键 词：DMBA 大豆异黄酮 塞来昔布 乳腺肿瘤 

分 类 号：R73-361[医药卫生—肿瘤]