茶多酚EGCG及UVB对角质形成细胞水通道蛋白3表达及EGFR／ERK信号传导途径的影响  

Effects of green tea polyphenol epigallocatechin-3-gallate and ultraviolet B on the expression of aquaporin3 and EGFR/ERK si^nalin2 pathway in keratinocytes

作　　者：宋秀祖[1] 许文[1] 相文忠[1] 许爱娥[1] 

机构地区：[1]杭州市第三人民医院皮肤科,310009

出　　处：《中华皮肤科杂志》2013年第2期117-120,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（81000697）;浙江省自然科学基金（Y2090843）;浙江省科技厅课题（2009C33148）

摘　　要：目的探讨茶多酚表没食子儿茶素没食子酸酯（EGCG）及中波紫外线（UVB）对角质形成细胞水通道蛋白3（AQP3）的表达及信号传导途径的影响。方法20例健康人每日外涂不同浓度EGCG乳膏，2周后测定皮肤含水量及经皮水分丢失（TEWL）变化。对培养的人角质形成细胞分别予以10-7，10-6，10-5mol／L的EGCG处理后予以UVB照射，或者用EGFR／ERK的磷酸化抑制剂处理后予以UVB照射，Western印迹检测AQP3蛋白表达变化以及EGFR／ERK信号传导途径变化。结果健康人皮肤予以不同浓度EGCG乳膏处理后，皮肤含水量显著增加，经皮水分丢失明显减少。UVB照射前予以10-7，10-6，10-5mol／LEGCG处理，AQP3表达明显升高，分别为172．36±12．42，320．66±15．51，368．10±11．39，与单纯UVB照射组（灰度值设为100．00）比较差异具有统计学意义（t值分别为12．16，26．75，38．62，P值均〈0．05）。UVB照射前予以EGFR磷酸化抑制剂PDl53035（1．0μmol／L）和ERK磷酸化抑制剂U0126（10μmol／L）处理后，AQP3表达也明显升高，分别为413．85±25．27，268．85±16．33，与单纯UVB照射组比较，差异具有统计学意义（t值分别为35．16，19．25，P值均〈0．05）。UVB照射可以激活角质形成细胞EGFR／ERK信号传导途径，预先予以EGCG处理可以显著抑制EGFR／ERK的磷酸化。结论EGCG可以增强皮肤屏障功能。UVB照射可以下调角质形成细胞AQP3表达，而EGCG处理可以上调AQP3表达，其机制可能与抑制UVB照射诱导的EGFR／ERK活化有关。Objective To evaluate the effect of green tea polyphenol epigallocatechin-3-gallate （EGCG） and ultraviolet B （UVB） on the expression of aquaporin 3 and epidermal growth factor receptor （EGFR）/ extracellular signal-regulated protein kinase （ERK） signaling pathway in keratinocytes. Methods Twenty healthy human subjects were enrolled in this study. Both legs of each subjects were separated into 4 areas to remain untreated （control area）, be topically treated with 3% and 1% EGCG cream and the vehicle of EGCG cream respectively once a day for 2 weeks followed by the measurement of skin moisture content and trans- epidermal water loss （TEWL）. Cultured keratinoeytes were classified into various groups to be irradiated with different doses （10, 20 and 30 mJ/cm2） of UVB, or be pretreated with different concentrations of EGCG （10-7, 10-6,10-5 mol/L） or EGFR/ERK phosphorylation inhibitors for 1 hour followed by irradiation with UVB of 30 mJ/cm2. After various durations of additional culture, Western blot was conducted to quantify the expression of AQP3 and phosphorylated-EGFR （p-EGFR） and -ERK （p-ERK） of keratinocytes. Data were processed by SPSS 10.0 software, and statistical analysis was carried out by t test. Results Skin moisture content was significantly increased, while TEWL was decreased in healthy skin after treatment with 1% and 3% EGCG cream compared with vehicle-treated skin areas and untreated skin areas. Increased AQP3 expression was observed in keratinocytes pretreated with EGCG of 10-7,104,10-5 mol/L （172.36 ± 12.42,320.66± 15.51 and 368.10 ± 11.39 vs. 100.00, t = 12.16, 26.75 and 38.62 respectively, all P 〈 0.05） and in those pretreated with the EGFR inhibitor PD153035 of 1.0μmol/L and ERK inhibitor U0126 of 10 μmol/L （413.85 ±25.27 and 268.85 ± 16.33 vs. 100.00, t = 35.16, 19.25 respectively, both P 〈 0.05）compared with those irradiated with UVB of 30 mJ/cm2alone. UVB irradiation stimulated the phosphorylation of EGFR/ERK in keratinocytes, and the s

关 键 词：皮肤屏障 水通道蛋白3 中波紫外线 表没食子儿茶素没食子酸酯 

分 类 号：R[医药卫生]