表没食子儿茶素没食子酸酯对A549细胞增殖、凋亡和hTERT表达的影响  

Effects of epigallocatechin-3-gallate on growth and hTERT expression of A549 cells

作　　者：张莎[1] 袁君[1] 张贵星[1] 张菊娥[1] 

机构地区：[1]郑州大学基础医学院生物化学与分子生物学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2012年第6期807-810,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨表没食子儿茶素没食子酸酯(EGCG)对人肺腺癌细胞株A549细胞增殖、凋亡及人端粒酶逆转录酶(hTERT)表达的影响。方法:用0、50、100、150、200、250mg/LEGCG分别作用A549细胞24、48和72h后,MTT比色法检测细胞增殖抑制率;0、100、200、250mg/LEGCG干预48h后,应用AnnexinV-FITC/PI双染法检测A549细胞的凋亡情况,应用RT-PCR和免疫细胞化学分别检测细胞内hTERTmRNA和蛋白的表达。结果:EGCG可抑制A549细胞的增殖,并随着剂量增加和时间延长,抑制率增加(F组间=185.944,F时间=291.234,F交互=4186.119,P均<0.001)。EGCG促进A549细胞凋亡(F=2757.183,P<0.001),还可抑制A549细胞中hTERTmRNA与蛋白的表达(F=260.861和953.327,P均<0.001)。结论:EGCG能有效地抑制A549细胞的增殖,诱导其凋亡,其机制可能与下调hTERT的表达有关。Aim：To investigate the effects of epigallocatechin-3-gallate（EGCG）on proliferation and apoptosis and the expression of human telomerase reverse transcriptase（hTERT）of human lung adenocarcinoma cell line A549.Methods：A549 cells were treated with 0,50,100,150,200,250 mg/L EGCG for 24,48,and 72 h,respectively.MTT assay was used to detect the inhibitory rate of cell proliferation.Flow cytometry with Annexin V-FITC/PI double staining was employed to analyze the apoptosis of A549 cells treated with various concentrations of EGCG（0,100,200,250 mg/L）for 48 h,and RT-PCR and immunocytochemistry were applied to detect the mRNA and protein expression of hTERT.Results：EGCG inhibited the cell proliferation of A549 in a dose dependent manner（Fgroup=185.944,Ftime=291.234,Finteraction=4 186.119,P0.001）,and promoted the apoptosis of A549 cells（F=2 757.183,P0.001）.EGCG could suppress the mRNA and protein expression of hTERT（F=260.861 and 953.327,P0.001）.Conclusion：EGCG can effectively inhibit cell proliferation and induce apoptosis of A549 cells,which may be associated with downregulation of hTERT expression.

关 键 词：表没食子儿茶素没食子酸酯 人端粒酶逆转录酶 肺肿瘤 A549细胞 

分 类 号：R734.2[医药卫生—肿瘤]