Improved biocompatibility of phosphorylcholine end-capped poly(butylene succinate)  

作　　者：ZHANG ShiPing WANG LiLi YANG Shan GONG YongKuan 

机构地区：[1]Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University [2]College of Chemistry and Life Science, Weinan Normal University

出　　处：《Science China Chemistry》2013年第2期174-180,共7页中国科学（化学英文版）

基　　金：financially supported by the National Natural Science Foundation of China (21004048, 20974087);the Natural Science Foundation of Shaanxi Educational Committe (2010JK886);the Science Foundation of Northwest University (NF0912)

摘　　要：In this work, the biocompatibility of a biomimetic, fully biodegradable ionomer phosphorylcholine (PC)-functionalized poly(butylene succinate) (PBS-PC) was investigated by means of hemolysis, platelet adhesion, protein adsorption and cytotox- icity experiments. The reference materials were poly(butylene succinate) (PBS) and chloroethylphosphoryl functionalized poly(butylene succinate) (PBS-Cl). The hemolysis rates (HR) of the leaching solutions of PBS, PBS-Cl and PBS-PC were all lower than the safe value, and the rate of PBS-PC was reduced to 1.07%. Scanning electron microscopy (SEM) measurements showed that platelet adhesion and aggregation were significant on both PBS and PBS-Cl surface. In contrast, very few platelets were observed on PBS-PC surface. Bicinchoninic acid (BCA) measurements revealed that the adsorption amounts of bovine serum albumin (BSA) and bovine plasma fibrinogen (BPF) on PBS-PC surface were 52% and 72% reduction respectively compared with those on PBS surface. Moreover, non-cytotoxicity of both PBS-PC particles and its leaching solution was sug- gested by MTT assay using mouse L929 fibroblast cells. All the results demonstrated that the biocompatibility of PBS could be greatly improved by PC end-capping strategy. This PC functionalized polyester may have potential applications in biological environments as a novel carrier for controlled drug release and scaffold for tissue engineering.In this work, the biocompatibility of a biomimetic, fully biodegradable ionomer phosphorylcholine （PC）-functionalized poly（butylene succinate） （PBS-PC） was investigated by means of hemolysis, platelet adhesion, protein adsorption and cytotox- icity experiments. The reference materials were poly（butylene succinate） （PBS） and chloroethylphosphoryl functionalized poly（butylene succinate） （PBS-Cl）. The hemolysis rates （HR） of the leaching solutions of PBS, PBS-Cl and PBS-PC were all lower than the safe value, and the rate of PBS-PC was reduced to 1.07%. Scanning electron microscopy （SEM） measurements showed that platelet adhesion and aggregation were significant on both PBS and PBS-Cl surface. In contrast, very few platelets were observed on PBS-PC surface. Bicinchoninic acid （BCA） measurements revealed that the adsorption amounts of bovine serum albumin （BSA） and bovine plasma fibrinogen （BPF） on PBS-PC surface were 52% and 72% reduction respectively compared with those on PBS surface. Moreover, non-cytotoxicity of both PBS-PC particles and its leaching solution was sug- gested by MTT assay using mouse L929 fibroblast cells. All the results demonstrated that the biocompatibility of PBS could be greatly improved by PC end-capping strategy. This PC functionalized polyester may have potential applications in biological environments as a novel carrier for controlled drug release and scaffold for tissue engineering.

关 键 词：PHOSPHORYLCHOLINE poly（butylene succinate） BIOCOMPATIBILITY platelet adhesion HEMOLYSIS CYTOTOXICITY 

分 类 号：O632[理学—高分子化学]