EGCG对16Hz，155dB次声诱导的大鼠小胶质细胞过度激活的影响  

Effects of EGCG on the excessive activation of rat microglia cells induced by infrasound with 16 Hz,155 dB

作　　者：蔡婧[1] 杜芳[1] 景达[2] 史明[1] 赵钢[1] 

机构地区：[1]第四军医大学 西京医院神经内科 [2]第四军医大学 生物医学工程学院军队卫生装备与计量学教研室,西安710032

出　　处：《神经解剖学杂志》2013年第1期40-44,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(31070756);陕西省科技攻关项目(2010K15-09-02)

摘　　要：目的:研究表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)对次声诱导的大鼠小胶质细胞过度激活的抑制作用,探讨EGCG潜在的次声对脑损伤的防护作用。方法:16 Hz,155 dB次声刺激原代培养的小胶质细胞,通过细胞因子表达量和免疫荧光染色,观察不同浓度(1μmol/L、10μmol/L、100μmol/L)的EGCG在不同时程(12 h和24 h)对次声激活的小胶质细胞的抑制作用。结果:1μmol/L或10μmol/L EGCG预孵可显著抑制次声后大鼠小胶质细胞IL-1β、IL-6、IL-18、TNF-α表达的上调,且10μmol/L组的作用最强(P<0.01)。10μmol/L EGCG可以减少次声诱导的小胶质细胞过度激活的形态变化。结论:EGCG对次声诱导的大鼠小胶质细胞过度激活具有显著的抑制作用。Objective： To investigate the inhibitive effects of epigallocatechin gallate （EGCG） on infrasound-induced excessive activation of rat microglia cells, and thus explore the potential protective role of EGCG on brain injures induced by infrasound. Methods： The primary rat microglia cells were exposed to the infrasound with 16 Hz, 155 dB. Cells were then incubated in 1 μmol/L, 10 μmoL/L or 100 μmol/L EGCG for 12 h or 24 h and the inhibitive effects of EGCG were evaluated via the immunofluorescence staining and cytokine expression determination. Results： EGCG with 1 μmol/L and 10 μmol/L significantly prevented the infrasound induced up-regulation of IL-1β, IL-6, IL-18, TNF-α, especially in 10 μmol/L EGCG group （P 〈 0.01 ）. 10 μmol/L EGCG group could also significantly reverse the morphological changes due to infrasound-induced excessive activation of microglia cells. Conclusion： EGCG demonstrated dramatic inhibitive effects on infrasound-induced excessive activation of rat microglia cells.

关 键 词：表没食子儿茶素没食子酸酯 次声 小胶质细胞 细胞因子 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]