LC-MS/MS同时测定动物源性食品中20种游离态性激素残留  

Simultaneous analysis the residues of 20 hormones in foods of animal origin by liquid chromatography tandem mass spectrometry

作　　者：刘桂华[1] 秦伟[2] 刘红河[1] 朱舟[1] 

机构地区：[1]深圳市疾病预防控制中心,深圳518055 [2]秦皇岛市疾病预防控制中心

出　　处：《卫生研究》2013年第1期61-66,共6页Journal of Hygiene Research

基　　金：2008年深圳市科技计划项目(No.200802077)

摘　　要：目的建立快速、准确的液相色谱串联质谱(LC-MS/MS)同时测定动物源性食品中多种性激素残留的确证方法,调查深圳市售猪肉、牛肉、鸡蛋和牛奶中激素残留情况。方法采用乙腈提取待测组分,经过正己烷去脂、HLB-NH2柱串联固相萃取净化后,采用LC-MS/MS电喷雾电离(ESI),多反应监测(MRM)模式检测,内标法定量。结果所测样品中大都检出一定量的内源性激素,20种目标性激素的方法定量限为0.5～1.0μg/kg,方法平均回收率60.4%～118.2%,相对标准偏差2.5%～16.2%。结论方法快速准确,可用于动物源性食品(猪肉、牛肉、鸡蛋和牛奶)中20种游离态性激素的检测。Objective To develop a quick and accurate method for simultaneous determining the multi-residues of hormones in foods of animal origin by liquid chromatography-tandem mass spectrometry （LC-MS/MS）, and to investigate these residues in swine, bovine, egg and milk collected from local markets in Shenzhen. Method The sample was firstly extracted with aeetonitrile, and then subjected to solidphase extraction clean-up using HLB-NH2 cartridges after defatted with hexane, lastly detected by high performance liquid chromatography-tandem mass spectrometry. Identification was achieved by electrospray ionization （ESI） in both positive and negative mode using multiple reaction monitoring. Quantification was performed by internal standard calibration. Results The study showed that there was a certain amount of endogenous hormones in collected samples. The limits of quantification were 0. 5 - 1.0 μg/kg for 20 hormones in swine, bovine, egg and milk. Average recoveries were 60. 4% - 118.2% , and the relative standard deviations were 2.5% - 16.2%. Conclusion This method was quick and accurate which could be used for determination of hormones in foods of animal origin （such as swine, bovine, egg and milk）.

关 键 词：动物源性食品 性激素 液相色谱串联质谱 残留 

分 类 号：TS207.5[轻工技术与工程—食品科学] R155.51[轻工技术与工程—食品科学与工程]