电压敏感荧光染料在鱼贝类毒素检测中的应用初探  

Application of a fluorescent dye method based on changes in membrane potential in detecting shellfish toxins

作　　者：高利娟[1] 杨维东[1] 李宏业[1] 刘洁生[1] 

机构地区：[1]暨南大学生命科学技术学院,广州510632

出　　处：《卫生研究》2013年第1期139-142,共4页Journal of Hygiene Research

基　　金：国家自然科学基金(No.40976065);广东省自然科学基金重点项目(No.8251063201000001);中央高校基本科研费

摘　　要：目的建立快速、简便、能反映样品整体毒性的鱼贝类毒素筛查方法。方法以神经母细胞瘤细胞为实验材料,采用电压敏感荧光染料bis-oxonol,根据膝沟藻毒素2,3(GTX2,3)、短裸甲藻毒素(BTX)和河豚鱼毒(TTX)的毒理特点,观察不同浓度毒素作用下细胞荧光的变化。结果 GTX2,3的浓度在2～200ng/ml范围内,TTX的浓度在20～600ng/ml范围内,BTX的浓度在15～400ng/ml范围内,培养体系荧光强度与毒素的浓度之间存在很好的线性关系。结论利用电压敏感荧光染料法可实现对GTX2,3、TTX和BTX的快速检测。Objective To develop a rapid and sensitive assay for detecting the common sea food toxins including paralytic shellfish poisoning toxin （PST） , tetrodotoxin （TTX） and neurotoxic shellfish poisoning toxin （NST） based on their toxicological character. Methods Neuroblastoma cells were incubated with the fluorescent dye bisoxonol, whose distribution across the membrane was potential-dependent. Changes in membrane potential of the cells induced by gonyautoxins （GTX2,3） , brevetoxin （BTX） and TTX were observed respectively, using bis-oxonol. Results Within 2 -200nmol/L of GTX2,3 or 20 -600nmol/L of TTX, veratridine-indueed depolarization was shown to be inhibited by GTX2,3 or TTX in dose-dependent manner. Within 15 -400ng/ml, there was a dos-dependent relationship between the NSP-induced depolarization and toxin concentration. Conclusion It was likely to find a rapid, specific, and reliable method with bis-oxonol for detecting GTX2,3, TTX and BTX in sea food.

关 键 词：麻痹性贝毒 河豚毒素 神经性贝毒 bis-oxonol 

分 类 号：R155.51[医药卫生—营养与食品卫生学] TS207.3[医药卫生—公共卫生与预防医学]