正丙醇提取茶皂素工艺  

Optimization of Extraction Process for Tea Saponin from Camellia oleifera Seed Meal with n-Propanol

作　　者：于辉[1] 陈海光[1] 吴波[1] 任文彬[1] 

机构地区：[1]仲恺农业工程学院轻工食品学院,广东广州510225

出　　处：《食品科学》2013年第2期58-62,共5页Food Science

基　　金：广东省教育部产学研结合项目(2009B090200028);广东省中国科学院全面战略合作项目(2010B090301010)

摘　　要：采用正丙醇浸提提取油茶籽粕中的茶皂素。考察原料颗粒的目数、浸提液正丙醇溶液的体积分数、料液比、浸提时间、浸提温度以及浸提液pH值对提取效果的影响,并用正交试验获得最佳工艺。以茶皂素得率为优化对象时,最佳工艺为脱脂茶粕60目、80%正丙醇、料液比1:12(g/mL)、温度80℃、时间3h,此条件下,茶皂素得率20.13%、纯度62.78%;以茶皂素纯度为优化对象时,最佳工艺为90%正丙醇、温度80C、脱脂茶粕40目、时间3h、料液比1:11,此条件下,茶皂素纯度72.06%、得率15.31%。上述工艺所得茶皂素产品的得率及纯度均优于常规乙醇浸提法。n-Propanol was used to extract tea saponin from oil tea （Camellia oleifera Abel.） seed meal. Operating parameters that influence extraction efficiency such as raw material particle size, n-propanol concentration, solid-to-solvent ratio, time, temperature and solvent pH were investigated by one-factor-at-a-time method and optimized using an orthogonal array design. The optimum extraction conditions for maximum yield of tea saponin were found to be 60 mesh, 80%, 1：12 （g/mL）, 80 ℃ and 3 h for raw material particle size, n-propanol concentration, temperatre and time, respectively, and the yield and purity of tea saponin obtained under these conditions were 20.13 % and 62.78%, respectively. The optimum extraction conditions for maximum purity of tea saponin were found to be 40 mesh, 90%, 1：11 （g/mL）, 80℃ and 3 h for raw material particle size, n-propanol concentration, temperature and time, respectively, and the yield and purity of tea saponin obtained under these conditions were 15.31% and 72.06%, respectively. However, lower yield and purity of tea saponin were obtained by the traditional ethanol extraction method.

关 键 词：茶皂素 正丙醇 提取 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]