响应面法优化超声波辅助提取湖北海棠叶中总黄酮工艺  

Optimization of Ultrasonic-Assisted Extraction Process for Total Flavonoids from Malus hupehensis Leaves by Response Surface Methodology

作　　者：乔孟[1] 屈晓清[1] 丁之恩[1] 

机构地区：[1]安徽农业大学茶与食品科技学院,安徽合肥230036

出　　处：《食品科学》2013年第2期143-147,共5页Food Science

摘　　要：采用超声波辅助提取湖北海棠叶中总黄酮,并利用响应面法对提取工艺参数进行优化。在料液比、乙醇体积分数、超声时间、超声功率等单因素试验基础上,进行四因素三水平的Box-Behnken设计,利用响应面法分析4个因素对湖北海棠叶中总黄酮得率的影响。超声波法提取湖北海棠叶中总黄酮的最佳工艺条件为液料比50:1(mL/g)、乙醇体积分数67.35%、超声时间3.2h、超声功率162.4W,总黄酮得率预测值为12.78%,验证值为12.76%,与理论值的相对误差为0.02%,表明该优化方法合理可行。Response surface methodology （RSM） was applied to optimize conditions for the ultrasonic-assisted extraction of total flavonoids from Malus hupehensis leaves. One-factor-at-a-time method was used to explore the effects of solvent- to-solid ratio, ethanol concentration, ultrasonic treatment time and ultrasonic power on extraction efficiency. Based on this, these process conditions were optimized using a four-variable, three-level Box-Behnken experimental design couple with response surface analysis to be 67.35% ethanol as the extraction solvent at a solvent-to-solid ratio of 50：1 （mL/g） and ultrasonic treatment for 3.2 h at 162.4 W. Under the optimized conditions, the predicted and actual yields of total flavonoids were 12.78% and 12.76% with a relative error of 0.02%. Therefore, the optimized extraction procedure is reliable.

关 键 词：湖北海棠叶 总黄酮 超声波辅助提取 响应面优化 

分 类 号：R284.2[医药卫生—中药学]