壳聚糖双胍盐酸盐抑菌剂的合成及在包装上的应用  

Synthesis of Antimicrobial Chitosan Biguanidine and Its Application in Packaging

作　　者：李灵[1] 王建清[1] 

机构地区：[1]天津科技大学,天津300222

出　　处：《包装工程》2013年第3期26-31,共6页Packaging Engineering

基　　金：国家"十二五"科技支撑项目:食品包装关键技术装备及材料开发与示范(2011BAD24B01)

摘　　要：以壳聚糖和双氰胺为原料,制备了应用于肉禽类包装的壳聚糖双胍盐酸盐抑菌剂。利用正交实验优化设计了制备壳聚糖双胍盐酸盐的工艺条件,发现当盐酸浓度为0.40 mol/L,反应官能团物质的量比为1∶5,反应时间为20 min时,产物产率达到92.96%。红外光谱证明,壳聚糖的氨基和双氰胺的氰基已进行了亲核加成反应,合成了双胍产物壳聚糖双胍盐酸盐;抑菌试验表明,壳聚糖双胍盐酸盐的抑菌性明显优于壳聚糖,且对金黄葡萄球菌的抑菌性优于对大肠杆菌的抑菌性。加入10%(质量分数)抑菌剂的聚乙烯醇复合膜,对大肠杆菌和金黄葡萄球菌的抑菌率分别达到95.67%和97.45%。Antimicrobial chitosan biguanidine was prepared for meat and poultry packaging using chitosan and dicyan- diamide as raw material. The preparation process of chitosan biguanidine was optimized using orthogonal experiment method. It was found that when HC1 concentration is 0.4 mol/L, mole ratio of functional group is 1 ： 5, and the reac- tion time is 20 min, the best yield is 92.96%. The product was characterized by FHR and the result showed that the nucleophilic addition reaction of the amino-group of chitosan and the cyano-group of dicyandiamide has been done and the product is chitosan biguanidine. The results of antibacterial test showed that the antibacterial activity of chitosan biguanidine is obviously better than chitosan, and also the antibacterial activity against S. aureus is better than E. coli. The inhibition rate of the antibacterial film with 10% chitosan biguanidine against E. coli and S. aureus is 95.67% and 97.45% respectively.

关 键 词：壳聚糖 双氰胺 抑菌性 

分 类 号：TB487[一般工业技术—包装工程] TS206.4[轻工技术与工程—食品科学]